Microscale Thermophoresis

项目摘要 我们建议获得NanoTemper Monolith NT-Auto，检测荧光标记的 分子和内在荧光。该工具为研究人员提供了最大的灵活性， 是波士顿地区唯一的同类乐器。Monolith仪器测量微尺度 热泳，其基于在监测变化的同时产生精确的温度梯度 在样品中沿着该梯度沿着迁移，如通过样品荧光检测以确定结合。的 分子沿着温度梯度迁移的变化反映了分子水合壳的变化， 电荷或大小;由于这些参数中的一个或全部在配体结合时改变，因此结合和 可以确定未结合状态。所要求的仪器允许测定结合亲和力， 低聚和其他参数而不固定。该仪器将用于推进 与癌症、抗生素耐药性、传染病、病毒复制、炎症 和神经系统疾病包括成瘾。第二个主要重点是药物设计，开发， 交付.

Project Summary We propose to acquire a NanoTemper Monolith NT-Auto, with detection of both fluorescently-labeled molecules and of intrinsic fluorescence. This instrument provides maximum flexibility for investigators and will be the only instrument of its kind in the Boston area. The Monolith instruments measure microscale thermophoresis, which is based on the generation of a precise temperature gradient while monitoring changes in sample migration along this gradient, as detected by sample fluorescence to determine binding. The changes in migration of molecules along the temperature gradient reflect changes in molecular hydration shell, charge, or size; as one or all of these parameters change upon ligand binding, differences in the bound and unbound states can be determined. The requested instrument allows determination of binding affinities, oligomerization, and other parameters without immobilization. The instrument will be used to advance numerous projects related to cancer, antibiotic resistance, infectious disease, viral replication, inflammation, and neurological disorders including addiction. A second major focus is on drug design, development, and delivery.