Analytical ultracentrifuge with absorbance and interference optics.

项目摘要/摘要 购买分析超速离心机(AUC)的资金，型号为Beckman Couture Optima AUC 被请求了。这一最先进的仪器将扩大NIH核心数量的研究能力 北达科他州立大学(NDSU)资助的研究人员。AUC将允许对 溶液中的大分子，以获得有关大小、形状、异质性、构象和 这些样品的组装动力学。这些生物物理信息提供了重要的信息 生物过程的激活、抑制、整体调节和一般功能。被选中的 仪器同时具有干涉光学和吸收光学，从而能够表征大范围的 样品类型，包括没有特征吸收系数的糖和脂。目前， NDSU没有这种仪器，半径200英里内也没有AUC。因此，用户是 目前依赖通过远程协作或付费服务提供商访问AUC。这可以是 对于调查人员来说，效率低下和/或昂贵得令人望而却步。仪器将被安置在一个核心设施中 由NDSU支持的全职博士科学家管理，他们的工资不依赖于用户费用。 因此，仪器的访问和维护将受到直接监督，以保护仪器的寿命 乐器。将解决的直接相关的项目涉及1)蛋白质：蛋白质相互作用 参与细菌细胞表面信号传递，2)了解激动剂/拮抗剂对蛋白质的影响：蛋白质 相互作用涉及G蛋白偶联受体，3)蛋白质：与自噬有关的蛋白质相互作用 调节，以及4)利用酶的血红素的组装动力学。总体而言，我们的用户组代表 三所大学四个系的研究人员，该仪器将促进各领域的研究 与药理学有关的材料。NDSU的AUC的出现将极大地促进研究 这些项目和其他与NIH相关的项目的方向。它将显著增加对学生的培训 教师的机会和研究效率。

PROJECT SUMMARY/ABSTRACT Funds to purchase an Analytical Ultracentrifuge (AUC), model Beckman Couture Optima AUC, are being requested. This state-of-the-art instrument will expand the research capabilities of a core number of NIH funded investigators at North Dakota State University (NDSU). The AUC will allow characterization of macromolecules in solution to obtain quantitative details on the size, shape, heterogeneity, conformation, and assembly dynamics of these samples. This biophysical information provides critical information to understand the activation, inhibition, overall regulation, and general function of biological processes. The chosen instrument has both interference and absorbance optics allowing for the characterization of a broad range of sample types, including sugars and lipids that do not have characteristic absorbance coefficients. Currently, NDSU does not have this instrumentation and there is no AUC within a 200 mile radius. Therefore, users are currently relying on access to an AUC via long distance collaboration or for fee service providers. This can be inefficient and/or prohibitively expensive for investigators. The instrument will be housed in a core facility managed by a full time Ph.D. scientist, supported by NDSU and whose salary is not dependent on user fees. Thus, access and maintenance of the instrument will have direct supervision protecting the longevity of the instrument. Projects of immediate relevance that will be addressed involve 1) protein:protein interactions involved in bacterial cell surface signaling, 2) understanding agonist/antagonist influence on protein:protein interactions involve in a G-protein coupled receptor, 3) protein:protein interactions involved in autophagy regulation, and 4) assembly dynamics of a heme utilizing enzyme. Overall, our user group represents investigators in four departments across three colleges and the instrument will facilitate research in areas related to pharmacology to materials. The presence of an AUC at NDSU will greatly enhance the research directions of these and other projects of NIH relevance. It will significantly increase student training opportunities and research productivity of faculty.