ShEEP Request for KEYENCE BZ-X800 All-in-One Fluorescence Microscope

项目摘要/摘要 Keyence BZ-x00是一个多功能、多功能的细胞和组织显微平台。它包含了 6个物镜，放大倍数从2倍到100倍不等。它可以用来在白光下成像 对培养中的整个组织切片和细胞进行组织学检查，并使用荧光成像滤光片 所有的颜色范围。本方案中的BZ-X800配置包括一个集成加热室 在附带的自动焦平面校正软件的帮助下，使用二氧化碳进行扩展的活细胞成像。它 还集成了可选的栅格系统，用于结构化照明，可以在光线照射到 样品可以去除焦外光线，并提供具有共焦显微镜分辨率的图像。任选 包括数据分析包，用于自动拼接大图像、混合细胞计数和3D 细胞和组织的重建。它从头开始设计为带有存储模块的独立系统 这取代了对暗室的需要，并用软件控制将用户出错的可能性降至最低 导致硬件故障。 ·Lee：Current VA Merit，5I01 BX003265-05，研究MIF-CD74信号在保护中的机制 通过激活特定的细胞存活、抗氧化剂和DNA修复分子和 衰老蛋白p16的抑制作用。 ·Lee：目前的国防部赠款PR150809正在测试MIF增强功能，以对抗与香烟烟雾暴露相关的 慢性阻塞性肺疾病及其并发症肺炎链球菌肺炎 ·Spurney：待定的VA功绩续签：I01 BX002984-05检查了促成因素的分子机制 糖尿病肾病对肾小球足细胞损伤的影响 ·斯普尔尼：国防部拨款(PR180545)和国家卫生研究院培训拨款(T32 DK007731)，是 正在执行R01拨款的调查员(R01-DK098135)。 ·米勒：目前的VA Merit，I01 BX001729正在研究慢性炎症的分子机制 加速血管疾病。研究是在培养的平滑肌细胞和一种小鼠模型上进行的。 颈动脉损伤。 ·米勒：未决的VA Merit，I01 BX005308，是IND前的一项研究，旨在检查合成RNA的安全性 用于治疗增生性血管疾病的配体。 ·米勒：I01 BX005088是一个为期四年的优点，它将识别选择性与炎症结合的RNA适配子 内皮细胞和另一种适体抑制平滑肌细胞的生长。研究是在培养的 细胞和血管疾病的小鼠模型。 ·米勒：I01 BX005450是一项为期两年的奖励计划，旨在确定细胞外的失活 由先前描述的合成RNA配体组成的组蛋白将保护急性白血病的发展 新冠肺炎中的呼吸窘迫综合征。这些研究是在仓鼠模型上进行的 新冠肺炎。 ·Pisetsky：Va Merit，5I01BX003772-02，SLE中自身抗原的细胞来源 ·麦克马洪：弗吉尼亚州梅里特，弗吉尼亚州BX-003478-01。SNO转运调节红细胞内皮细胞黏附。我们的目标是 确定红细胞输出和内皮细胞输入SNO的机制，形成信号转导 路径。 ·泰格：该实验室对空气污染改变肺部结构和功能的机制很感兴趣。 ·默多克：我们的研究涉及慢性炎症对合并症和终末性器官疾病的影响， 包括呼吸道疾病和药物滥用引发的炎症。

Project Summary / Abstract The Keyence BZ-X00 is a multi-functional and versatile microcopy platform for cells and tissues. It incorporates 6 objective lenses with magnification power from 2X up to 100X. It can be used to image with white light for histological examination of whole tissue slices and cells in culture, and by using filters for fluorescence imaging across the color spectrum. The BZ-X800 configuration in this proposal includes an integrated heating chamber with CO2 for extended live-cell imaging, facilitated by the included automatic focal plane correction software. It also incorporates an optional grid system for structured illumination that can be inserted before the light hits the sample to remove out of focus light and provide images with the resolution of confocal microscopes. Optional data analysis packages are included for automated stitching of large images, hybrid cell counting and 3D- reconstructions of cells and tissues. It is designed from the ground up as a stand-alone system with an enclosure that replaces the need for a dark room and with software controls that minimize the possibility of user errors leading to hardware malfunctions. • Lee: Current VA Merit, 5I01 BX003265-05, examining mechanisms of MIF-CD74 signaling in protecting against emphysema through activation of specific cell survival, antioxidant, and DNA repair molecules and the suppression of senescence protein p16. • Lee: Current DoD Grant, PR150809, is testing MIF augmentation against cigarette smoke exposure-related COPD and its subsequent complication, Streptococcus pneumoniae pneumonia • Spurney: Pending VA Merit Renewal: I01 BX002984-05 examines the molecular mechanisms that contribute to injury of glomerular podocytes in diabetic kidney disease • Spurney: Department of Defense grant (PR180545) and an NIH training grant (T32 DK007731) and is a co- investigator on an active R01 grant (R01-DK098135). • Miller: Current VA Merit, I01 BX001729, is examining molecular mechanisms by which chronic inflammation accelerates vascular disease. Studies are performed in cultured smooth muscle cells and a murine model of carotid injury. • Miller: Pending VA Merit, I01 BX005308, is a pre-IND enabling study to examine the safety of Synthetic RNA ligands for the treatment of hyperproliferative vascular disease. • Miller: I01 BX005088 is a four-year Merit that will identify RNA aptamers that selectively bind to inflamed endothelial cells and another aptamer to inhibit smooth muscle cell growth. Studies are performed in cultured cells and murine models of vascular disease. • Miller: I01 BX005450 is a two-year Merit that proposes to determine whether inactivation of extracellular histones by a previously described synthetic RNA ligand will protect from the development of acute respiratory distress syndrome (ARDS) in COVID-19. These studies are performed in hamster models of COVID-19. • Pisetsky: VA Merit, 5I01BX003772-02, Cellular Sources of Self Antigen in SLE • McMahon: VA Merit, VA BX-003478-01. SNO transport regulates endothelial adhesion of RBCs. The goal is to determine the mechanism by which RBCs export and endothelial cells import SNOs, forming a signaling pathway. • Tighe: The laboratory is interested in mechanisms by which air pollution alters lung structure and function. • Murdoch: Our studies relate to the impact of chronic inflammation on comorbidities and end organ disease, including airway disease and substance abuse-driven inflammation.