Effect of piezoelectric charges on oral microbiome modulation

项目总结 继发性龋病被认为是导致牙齿修复失败的主要原因。中国的经济负担 在美国，治疗这种牙病意义重大，每年花费超过50亿美元。提高产品的密封性 粘结在牙齿硬组织上的牙科材料对于预防继发性龋齿的进展至关重要。 牙病和牙齿脱落的风险。清除粘合界面上的细菌将防止(生物)化学物质 粘结材料的降解，从而增加修复体的临床服务。一种常见的 当前抗菌技术的局限性包括淋溶和抗菌作用的持久性较短 离子的耗尽。因此，迫切需要开发新一代牙科抗菌剂。 减少和消除粘接界面上的牙病(如继发性龋齿)的材料。我们最近 通过掺入带电材料开发了一种具有压电性能的抗菌治疗新复合材料 在牙科树脂中加入纳米填充物。我们的创新方法显著改进了当前的技术，通过使用 一种具有持久抗菌效果的单一填充物。在这项研究中，我们建议找到最佳的 压电纳米填料能够在保持高机械和物理性能的同时达到抗菌效果 复合材料的属性(目标1)。此外，我们还将评估其对口腔微生物群的调节作用 了解压电荷电对生物膜组成的具体变化并确定靶标 通过压电式电荷传播物种(目标2)。我们预计拟议技术的发展将 通过降低复合修复体的失败率显著影响牙科领域，进而减少 与这些故障相关的成本，改善患者的健康，并防止不必要的 附加的齿结构。

PROJECT SUMMARY Secondary caries is considered the leading cause of dental restorations failure. The economic burden of treating this dental condition is significant at a cost of over $5 billion each year in USA. Improving the seal of dental materials bonded to dental hard tissues is critical for the prevention of secondary caries, progression of dental disease and tooth loss. Removing bacteria at the bonded interface will prevent the (bio)chemical degradation of the bonded materials, and thus, increase the clinical service of restorations. A common limitation of current antibacterial technologies includes the short durability of the effects due to leaching and depletion of ions. Therefore, there is an immense need to develop a new generation of dental antibacterial materials to reduce and eliminate dental diseases at bonded interfaces (e.g. secondary caries). We recently developed a new composite with piezoelectric properties for antibacterial therapies by incorporating charged nano-fillers into dental resins. Our innovative approach significantly improves current technologies by using a single filler with long-lasting antibacterial effects. In this study, we propose to find the optimal amount of piezoelectric nano-filler to enable the antibacterial effect while retaining high mechanical and physical properties of the composite (aim 1). In addition, we will evaluate the oral microbiome-modulating effects of piezoelectric charges to understand the specific changes on the biofilm composition and identify the targeted species by piezoelectric charges (aim 2). We expect the development of the proposed technology to significantly impact the dental field by reducing the failure rate of composite restorations, in turn, reducing the costs associated with those failures, improving the health of patients, and preventing unnecessary loss of additional tooth structure.