National Resource for Aplysia

摘要 加利福尼亚海兔是一种独特的动物模型，对基本的 神经生理学以及记忆和学习的细胞基础。海兔的基因组已经被 测序和广泛的基因组和转录组资源是可用的，促进了广泛的多样性 基因表达研究。海兔也是研究衰老的杰出模型，每年 生命周期以显著的行为、神经生理和转录特征进展为标志 致衰老和死亡。国家海兔资源中心的总体目标是 迈阿密大学(The Resources)是生产和分销始终如一的高质量培养物 加州海兔，A.calfornica，用于生物医学研究和教育的所有生命阶段，以及 改进和扩大这一模式系统对生物医学界的有用性。这样做的目的是 补充支持的申请是为了调查一种疾病的分布和潜在的病理 新发现的自然发生的海兔NidVirus感染，即深水海兔病毒1型(AAbV)。 对AAbV的调查与父母P40赠款的目标直接相关：目标1的关键组成部分 父母赠款的目的是监测动物的健康状况，并为此而限制 在过去的一年里，已经开始对孵化动物中的AAbV进行监测(初步 此处提供的数据)。因此，这里提出的实验显然符合《公约》目标1的范围。 家长助学金。拟议的研究将使我们能够解决关于以下方面的重要生物安全问题 AAbV的分布和效果不能仅靠父母的资助来支持。我们建议 两个主要目标：1.发展改进的检测和无AAbV的动物；2.评估 与AAbV相关的病理学。在目标1中，我们将开发几种非侵入性监测方法 海兔体内AAbV水平的变化包括血淋巴样本、鳃拭子、粪便样本和水样。 这些方法将使我们能够在扑杀感染动物的同时识别队列中未感染的动物。一个 此外，这一目标的主要目标将是创造持续和可靠的无AAbV(SPF)供应 动物。目标2将遵循三种不同的方法来了解AAbV感染的病理 检测与高病毒载量相关的基因表达和组织超微结构的变化。在这 目的探讨AAbV对胚胎和幼虫发育的影响。

Abstract Aplysia californica is a unique animal model contributing to groundbreaking discoveries in basic neurophysiology as well as the cellular basis of memory and learning. The genome of Aplysia has been sequenced and extensive genome and transcriptome resources are available, facilitating a wide variety of gene expression studies. Aplysia is also an outstanding model for studies of aging, with an annual lifecycle marked by significant behavioral, neurophysiological and transcriptomic hallmarks progressing to senescence and death. The overarching goals of the National Resource for Aplysia at the University of Miami (the Resource) are to produce and distribute consistently high-quality cultured California sea hares, A. californica, at all life stages for biomedical research and education, and to improve and expand the usefulness of this model system to the biomedical community. The goal of this application for supplemental support is to investigate the distribution and potential for pathology of a newly discovered, naturally occurring, nidovirus infection in Aplysia, Aplysia abyssovirus 1 (AAbV). Investigation of AAbV is directly relevant to the aims of the parent P40 grant: a key component of aim 1 of the parent grant is to monitor the health status of animals at the resource and to this end, limited monitoring of AAbV in hatchery animals has already been underway for the past year (with preliminary data presented here). Thus, the experiments proposed here clearly fit within the scope of aim 1 of the parent grant. The proposed studies would allow us to address important biosecurity concerns regarding the distribution and effects of AAbV that could not be supported by the parent grant alone. We propose two primary aims: 1. Development of improved testing and AAbV-free animals and 2. Assessment of pathology associated with AAbV. In aim 1 we will develop several methods for non-invasive monitoring of AAbV levels in Aplysia including hemolymph samples, gill swabs, fecal samples, and water samples. Theses methods will allow us to identify uninfected animals in cohorts while culling infected animals. An additional, major goal of this aim will be to create a consistent and reliable supply of AAbV-free (SPF) animals. Aim 2 will follow three discrete approaches to understand the pathology of AAbV infection by examining changes in gene expression and tissue ultrastructure associated with high viral loads. In this aim we will also investigate developmental impacts of AAbV on embryos and larvae.