National Resource for Aplysia

海兔国家资源

• 批准号: 7848633
• 负责人: MICHAEL C SCHMALE
• 金额: $ 8.21万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-13 至 2010-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7848633
• 关键词: National; Resource; Aplysia

DESCRIPTION (provided by applicant): This proposal describes the continuation of the National Resource for Aplysia, whose overall goal is to provide consistently high-quality cultured sea hares Aplysia californica (and their cultured red algal food) to researchers. A. californica is an important non-vertebrate (Opisthobranch mollusk) model system for health- related research, primarily in the neurophysiology of behavior and learning. We will continue to produce animals for research and to conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-projects/specific aims: 1. Production - Sales of animals to researchers in the US and internationally is projected to continue at present levels of 25,000 to 30,000 animals per year. This resource makes available high-quality A. californica at all life stages from eggs through mature adults by rearing animals in carefully controlled laboratory conditions. All life-stages are available in sufficient quantities to meet user demand throughout the year, eliminating reliance on wild-collected animals which have limited seasonal availability and are of unknown age and health status. 2. Production research programs - The resource has been continuously improved over the past 12 years by research to optimize rearing conditions for most life stages of A. californica. The proposed research program will focus on measurement and improvement of egg quality. 3. Animal Health Monitoring - We will continue a monitoring program based on screening animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animals at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied. 4. Developmental Neurophysiology - We will begin to develop Aplysia as an aging model by studying the changes in D-Aspartate- and other agonist-activated ionic currents that modulate sensory neuron input in the tail withdrawal reflex, using single cell voltage clamping and recording of the intact reflex. Through this combination of production and basic research, we will improve the model system and extend its usefulness to other areas of research. PUBLIC HEALTH RELEVANCE STATEMENT (provided by applicant): The California sea hare, Aplysia californica, is a large marine snail with a very simple nervous system. The National Resource for Aplysia raises and ships 25,000 to 30,000 of these animals per year to labs in the US and around the world for conducting basic research in neurobiology and behavior. This proposal would continue the production of these animals and expand their usefulness concerning studies of aging and Alzheimer's disease. D-Aspartate

描述(由申请人提供)：本提案描述了国家海兔资源的延续，其总体目标是向研究人员提供持续高质量的养殖海兔(及其养殖的红藻食物)。甲藻是一种重要的非脊椎动物模型系统，主要用于行为和学习的神经生理学的健康相关研究。我们将继续生产用于研究的动物，并进行基础研究，旨在探索新的模式用途和改善资源。有四个子项目/具体目标： 1.生产--向美国和国际上的研究人员出售动物预计将继续保持目前每年25,000至30,000只动物的水平。这一资源通过在严格控制的实验室条件下饲养动物，在从卵子到成熟成虫的所有生命阶段都可以获得高质量的A.calfornica。所有生命阶段都有足够的数量来满足用户全年的需求，从而消除了对季节性供应有限、年龄和健康状况未知的野生动物的依赖。 2.生产研究计划--在过去的12年里，通过研究优化大多数生命阶段的饲养条件，资源得到了不断的改进。拟议的研究计划将侧重于鸡蛋质量的测量和改进。 3.动物健康监测-我们将继续实施基于筛选动物健康参数的监测计划，以确保快速检测和完整记录孵化场动物在任何发育阶段可能发生的任何疾病过程。对观察到的任何疾病症状和疑似病原体进行调查，并采取适当的控制措施。 4.发育神经生理学-我们将通过研究D-天冬氨酸和其他激动剂激活的离子电流的变化，利用单细胞电压钳制和记录完整的反射，开始发展海兔作为衰老模型。 通过这种生产和基础研究的结合，我们将完善模型体系，并将其实用性扩展到其他研究领域。 公共卫生相关声明(由申请人提供)：加州海兔，加利福尼亚海兔，是一种大型海洋蜗牛，神经系统非常简单。国家海兔资源中心每年饲养25,000到30,000只这种动物，并将其运往美国和世界各地的实验室，用于进行神经生物学和行为的基础研究。这项提议将继续生产这些动物，并扩大它们在衰老和阿尔茨海默病研究中的用途。D-天冬氨酸