Component for Institution # 264313

分子生物学核心是NIDA P30中心的关键组成部分 大学分子核心为研究人员提供服务，涉及短期或长期- 分子技术的使用。该核心提供三种类型的服务。第一，核心 支持设计和构建用于表达以下基因的分子构建体： 兴趣这将包括，例如，阿片样物质或阿片样物质受体的体外或体内表达。 大麻素受体这些分子构建体将允许各种感兴趣的研究， 合作研究人员，包括受体介导的信号传导过程的分析， 分析受体（或其他感兴趣的基因）的细胞运输。二是 分子生物学核心将提供体外和体内的基因表达分析。核心 使用RT-PCR、北方PCR和RT-PCR提供了mRNA和miRNA表达的高度定量分析。 印迹分析，RNA酶保护分析，以及新的更新应用，新的FISH PrimeFlow分析。核心还提供全面的转录因子功能 分析。第三，核心为动物核心和其他动物提供基因分型服务。 研究人员培育变异动物。服务对象是跨 各种学科和工作都是与调查员直接合作进行的。的 分子生物学核心特别感兴趣的是继续努力，以促进使用 利用分子技术研究滥用药物在宿主-寄生虫相互作用中的作用， 艾滋病。核心已经非常成功地吸引了来自许多国家的NIH资助的研究人员 在合作研究项目中的学科，已经推进了对 滥用药物的作用及其与免疫系统和艾滋病毒的相互作用。通过 将调查人员的专业知识与不同的兴趣、新的视角和潜在的 可以开展新的调查。

The Molecular Biology Core is a critical component of the NIDA P30 Center at Temple University. The Molecular Core offers services to investigators that involve either short- or long- term use of molecular techniques. This Core provides three types of services. First, the Core supports the design and construction of molecular constructs for the expression of genes of interest. This will include, for example, the expression either in vitro or in vivo of opioid or cannabinoid receptors. These molecular constructs will permit a variety of studies of interest to collaborating investigators including the analysis of receptor-mediated signaling processes and the analysis of the cellular trafficking of receptors (or other genes of interest). Second, the Molecular Biology Core will provide gene expression analysis both in vitro and in vivo. The Core provides highly quantitative analysis of mRNA and miRNA expression using RT-PCR, northern blot analysis, RNase protection analysis, and new to this renewal application, the novel FISH flow (PrimeFlow) analysis. The Core also offers comprehensive transcription factor function analyses. Third, the Core provides genotyping services for the Animal Core and to other investigators breeding mutant animals. Services are provided for investigators who span a variety of disciplines and work is carried out in direct collaboration with the investigator. The Molecular Biology Core is particularly interested in continuing efforts to promote the use of molecular techniques to study the role of drugs of abuse in the host-parasite interaction with HIV. The Core has been highly successful in engaging NIH-funded investigators from many disciplines in collaborative research projects that have advanced the understanding of the actions of drugs of abuse and their interplay with the immune system and with HIV. By combining the expertise of investigators with diverse interests, new perspectives and potentially novel investigations can be developed.