Flourescence-Activated Cell Sorter

描述（由申请人提供）：我们正在申请资金，将允许购买Becton Dickinson流入荧光激活细胞分选仪。该仪器将配置有生物安全柜，其将允许分选和/或分析原代人细胞群以及原代细胞和细胞系，其已经用各种病毒载体转导。该仪器还将配置为使用多达11种颜色和总共13个参数进行分类和/或执行多参数分析。本申请中描述的NIH资助的研究项目都需要访问具有这些功能的仪器。此外，该仪器将进一步加强我们的努力，扩大我们的NIH资助的研究企业。该仪器将允许进行至少28个NIH RO 1赠款或计划项目的一部分，由坦普尔大学医学院基础科学和临床部门的研究人员进行。这款顶级的多功能数字信号处理细胞分选仪将能够对原代人白细胞、神经元前体细胞、内皮细胞、成骨细胞、树突状细胞、造血干细胞、各种实体瘤细胞群、白血病和许多转导细胞系进行流式细胞术。包括NIH资助的项目将使我们能够开展直接相关的研究，以提高我们对广泛疾病的理解，包括癌症，动脉粥样硬化和对一些传染病的抵抗力，包括艾滋病毒感染。

DESCRIPTION (provided by applicant): We are requesting funds that will permit the purchase of a Becton Dickinson Influx Fluorescence Activated Cell Sorter. This instrument will be configured with a biological safety cabinet that will permit the sorting and/or analysis of primary human cell populations, as well as primary cells and cell lines, which have been transduced with various viral vectors. This instrument will also be configured to sort and/or perform multiparmetric analysis using up to 11 colors, and a total of 13 parameters. The NIH funded research projects described in this application all require access to an instrument that has these capabilities. In addition the instrument will further bolster our efforts to expand our NIH-funded research enterprise. This instrument will allow work to be carried out which is part of at least 28 NIH RO1 grants or Program Projects, by investigators in both basic science and clinical departments at Temple University School of Medicine. This top of the line versatile digital signal processing cell sorter will make it possible to carry out flow cytometry with primary human leukocytes, neuronal precursor cells, endothelial cells, osteoblasts, dendritic cells, hematopoietic stem cells, various solid tumor cell populations, leukemias, and a number of transduced cell lines. The NIH-funded projects that are included will make it possible to carry out research directly relevant to improving our understanding of a wide spectrum of diseases including cancer, atherosclerosis, and resistance to a number of infectious diseases, including infection with HIV.