Gas Chromatography-Molecular Rotational Resonance Spectrometer

气相色谱-分子旋转共振光谱仪 NIH SBIR直接进入II期 Jersey，Inc. 项目摘要 该项目提出了第一个商业气相色谱-分子的发展 用于复杂混合物分析表征的旋转共振（GC-MRR）光谱仪 例如生物流体和组织、天然产物或环境水源。MRR是一个 用于小分子化合物明确结构测定和定量的光谱技术 分子，它已经在学术研究实验室中使用了几十年，但直到最近才开始使用。 在最近突破性创新的推动下，关键优势 MRR促使其并入气相色谱联用系统的原因是其 对异构体化合物的选择性、绝对定量能力和 没有参考标准的未知数。GC-MRR不仅补充了现有技术（如 质谱和核磁共振），而且还增加了新的见解，以提高发现在许多 研究领域。 一个概念验证原型GC-MRR仪器已被用来证明其能力 解析复杂的混合物，包括共洗脱同位素体和同位素异构体。这 初步工作已经降低了拟议的第二阶段项目的风险，在第二阶段，将对原型进行改进 开发一种具有必要的灵敏度、速度和易用性的产品， 商业收养。这包括快速、精确测量的仪器硬件 当分析物从色谱柱中排出时，分析物的MRR光谱，以及软件 界面，使用户能够轻松地设置测量，可视化数据，并解释结果。的 然后，将在GC-MRR适用的市场区域对相关样品进行彻底测试。 预计将产生最大的影响。最后，经过广泛的评估， 我们会在资助期结束时或之前改良设计，以便投放市场。 完成的GC-MRR产品有望在许多领域实现突破性研究。 高影响力的健康相关领域，包括代谢组学，天然产物药物发现， 监测环境污染物等。

Gas Chromatography-Molecular Rotational Resonance Spectrometer NIH SBIR Direct to Phase II BrightSpec, Inc. Project Summary This project proposes the development of the first commercial gas chromatography-molecular rotational resonance (GC-MRR) spectrometer for analytical characterization of complex mixtures such as biological fluids and tissues, natural products, or environmental water sources. MRR is a spectroscopic technique for unambiguous structure determination and quantitation of small molecules, which has been used for decades in academic research laboratories but only recently commercialized by BrightSpec, driven by recent groundbreaking innovations. The key advantages of MRR motivating its incorporation into a gas chromatography hyphenated system are its selectivity to isomeric compounds, absolute quantitation capabilities, and identification of unknowns without reference standards. GC-MRR not only complements existing techniques (such as mass spectrometry and NMR), but also adds new insights to enhance discovery in numerous fields of research. A proof-of-concept prototype GC-MRR instrument has been used to demonstrate its capabilities to resolve complex mixtures, including co-eluting isotopologues and isotopomers. This preliminary work has de-risked this proposed Phase II project, where this prototype will be refined to develop a product with the sensitivity, speed, and ease-of-use necessary to facilitate its commercial adoption. This includes both the instrument hardware to quickly and precisely measure MRR spectra of analytes as they elute from the chromatographic column, and the software interface to allow users to easily set up measurements, visualize data, and interpret the results. The product will then be tested thoroughly on relevant samples in the market areas where GC-MRR is expected to have the greatest impact. Finally, following this extensive evaluation, the product design will be refined for commercial launch at or before the end of the grant period. The completed GC-MRR product holds the promise to enable breakthrough research in a number of high-impact health-related fields, including metabolomics, natural products drug discovery, and monitoring of environmental pollutants, among others.