BRAIN Viral Vector Services and Distribution Core

大脑病毒载体服务和分发核心将作为维护的资源， 神经科学家用于神经回路的病毒载体的传播和分布 识别和操作，以及临床前的翻译研究。高质量的病毒 载体是标记和操纵活体动物神经元回路以及识别长时间细胞周期的重要工具。 距离突触连接。此外，腺相关病毒(AAV)载体是事实上的 基因治疗的标准。现有的商业病毒载体来源不提供端到端的 服务，从协助病毒载体设计、严格制造和质量控制， 与客户一起进行故障排除，以增强大脑中的矢量性能，并专注于 神经科学。核心总监(Kim Ritola博士)将把她十年来提供病毒式工具的经验转化为 霍华德·休斯医学研究所神经科学界朝着创造脑病毒载体的方向发展 能够生产和分发AAV、狂犬病和Lenti病毒工具的服务和分销核心 对神经学家来说。核心将有两个主要目的：1)制作和传播负担得起的、 向神经科学界提供高质量的AAV、狂犬病和Lenti病毒载体，包括新的 开发载体、定制准备和管理库存以及2)与大脑研究人员合作 优化载体设计和准备条件以获得最佳性能，并作为教育 生产病毒载体的资源。这一核心将为调查人员提供广泛的选择 载体衣壳或包膜，与定制病毒结构配对，包括那些传统上难以识别的病毒 包装适合实验类型的基因组、效价和体积，严格的质量控制， 和可负担性。

The BRAIN Viral Vector Service and Distribution Core will serve as a resource for the maintenance, propagation, and distribution of viral vectors that are used by neuroscientists for neural circuit identification and manipulation, as well as for preclinical translational studies. High-quality viral vectors are essential tools to mark and manipulate neuronal circuits in live animals and identify long- range synaptic connections. Moreover, adeno-associated virus (AAV) vectors are the de facto standard for gene therapies. Existing commercial viral vector sources do not offer end-to-end services, from assisting with viral vector design, rigorous manufacturing and quality controls, troubleshooting with clients to enhance vector performance in the brain, and a mission focused on neuroscience. The Core Director (Dr. Kim Ritola) will translate her ten years of providing viral tools to the Howard Hughes Medical Institute neuroscience community towards creating a BRAIN Viral Vector Service and Distribution Core capable of producing and distributing AAV, rabies, and lenti virus tools to neuroscientists. The Core will have two main purposes: 1) Produce and disseminate affordable, high-quality AAV, rabies, and lenti viral vectors to the neuroscience community including newly developed vectors, custom preps and curated stocks and 2) Collaborate with BRAIN researchers to optimize vector design and prep conditions for optimal performance and serve as an educational resource for viral vector production. This Core will provide investigators with an extensive choice of vector capsid or envelope, paired with custom viral construct, including those with traditionally difficult to package genomes, titers and volumes appropriate for experiment type, rigorous quality controls, and affordability.