Advanced Analytics Core

高级分析核心 项目总结 高级分析(AA)核心提供三类主要的高级分析服务：单细胞 基因组学、免疫技术和Epcultures(新)。Epculate是源于有机或平面的培养物 来自捐赠者人类或模型生物的肠道。这些服务本质上是复杂的， 实验室独立执行的成本很高，而且需要高水平的技术培训。AA核心 一直专注于开发创新实践，以降低进入这些尖端技术的门槛 技术。虽然看起来不同，但这些服务已经被组合在一起，因为 管理专业知识、咨询实践和高效的商业模式实践。核心提供了 以大幅降低的成本和非常快的周转时间提供服务。AA Core的领导是 Scott Magness博士说，他被认为是单细胞基因组和功能分析的领导者。他有 在肠道和结肠类器官和使用初级组织来源的自我更新单层培养方面的专业知识 来源于人类和其他模式生物。AA核心人员都是训练有素的专业人员 这些对技术要求很高的方法，使AA Core在全国范围内获得了单电池的认可 测序服务。AA Core的具体目标是：1)提供最先进的单细胞分析 在细胞水平上更好地了解疾病/损伤过程和上皮再生。这些化验 包括荧光激活细胞分选(FACS)、单细胞(SC)RNAseq、DNAseq、miRNA表达 和单细胞免疫印迹。2)提供检测生物分子的免疫分析技术 通过酶联免疫吸附试验和以微球为基础的多重检测。通常，CGIBD成员使用这些检测来检测 与各种疾病状态相关的生物标记物，如IBD。3)提供与有机化合物相关的服务 上皮单层培养(Epcultures)，定制有机物分析，以及Epcultures中的培训。总而言之， 它们是强大的新技术，可以提供高度生理相关的体外模型来研究 影响胃肠道的各种健康状况。

ADVANCED ANALYTICS CORE PROJECT SUMMARY The Advanced Analytics (AA) Core provides three main classes of advanced analytics services: Single-cell genomics, Immunotechnologies, and EpCultures (new). EpCultures are organoid or planar cultures derived from the intestines of donor humans or model organisms. These services are sophisticated in nature, expensive for laboratories to independently perform, and require a high level of technical training. The AA Core has always focused on developing innovative practices to reduce barriers of entry to these cutting-edge technologies. While seemingly disparate, the services have been grouped together because of common management expertise, consultation practices, and efficient business model practices. The core provides services at substantially reduced costs and very rapid turnaround times. The AA Core is under the direction of Scott Magness, PhD, who is considered a leader in single-cell genomic and functional assays. He has expertise in intestinal and colonic organoid and self-renewing monolayer culture using primary tissue sources derived from humans and other model organisms. The AA Core personnel are highly trained professionals in these technically demanding methods, and have made the AA Core nationally recognized for single-cell sequencing services. The Specific Aims of the AA Core are: 1) To offer state-of-the-art single-cell assays to better understand disease/injury processes and epithelial regeneration at cell-level resolution. These assays include, Fluorescence Activated Cell Sorting (FACS), single-cell (sc) RNAseq, DNAseq, miRNA expression, and single-cell Western blotting. 2) To provide immunoassay technologies for the detection of biomolecules via ELISA and bead-based multiplex assay. Typically, CGIBD members use these assays to detect biomarkers associated with various disease states, such as IBD. 3) To offer services related to organoid and epithelial monolayer cultures (EpCultures), custom organoid assays, and training in EpCultures. Collectively, they are powerful new technologies that provide highly physiologically relevant ex vivo models to study a variety of health conditions that affect the GI tract.