Molecular Genetics Core

项目概要/摘要-分子遗传学核心 鉴于模式生物和分子遗传学工具对糖尿病及其并发症研究的价值， 分子遗传学核心（MGC）旨在帮助糖尿病研究人员开发 新的啮齿动物模型和分子工具，以确定细胞和分子机制， 糖尿病MGC成立于2015年，是一个收费服务核心，促进了分子生物学的应用。 遗传学方法应用于糖尿病相关研究。具体来说，MGC（1）设计并产生基因- 用于糖尿病相关研究的改良啮齿动物模型（使用CRISPR/Cas9）;（2）设计和生产 用于糖尿病研究的AAV载体;（3）生产和提供用于糖尿病研究的专用病毒试剂。 糖尿病研究;（4）为MDRC成员提供使用这些技术的建议和培训 laboratories. MG Core还拥有并维护多个共享设备，供MDRC使用 成员分布在不同地点周围的UM医学校园。 虽然CRISPR/Cas9技术已经大大提高了这种方法的速度并降低了成本， 模型可以生成，这种新技术不断发展的步伐可以预防许多糖尿病 研究人员充分利用其潜力。MGC利用其专业知识填补了这一空白， 设计和构建CRISPR/Cas9靶向试剂的人员，与UM转基因核心合作 在胚胎中测试这些试剂并产生创始小鼠，并识别用于转移的创始小鼠（沿着 基因分型方案）提供给MDRC研究者。为了产生病毒试剂，MGC设计并 产生任何必要的构建体，其通过UM病毒载体核心包装成病毒。与输入 从MDRC成员和MGC咨询委员会，MGC还确定和开发新的 技术（病毒和遗传），以支持MDRC成员的研究计划。

Project Summary/Abstract - Molecular Genetics Core Given the value of model organisms and molecular genetic tools for the study of diabetes and its co- morbidities, the Molecular Genetics Core (MGC) is designed to aid diabetes researchers in the development of novel rodent models and molecular tools to determine the cellular and molecular mechanisms contributing to diabetes. Established in 2015, the MGC is a fee-for-service core that facilitates the application of molecular genetic methods to diabetes-related research. Specifically, the MGC (1) designs and produces genetically- modified rodent models (using CRISPR/Cas9) for use in diabetes-related research; (2) designs and produces AAV vectors for use in diabetes research; (3) produces and provides specialty viral reagents for use in diabetes research; and (4) provides advice and training in the use of these technologies to members of MDRC laboratories. The MG Core also owns and maintains several pieces of shared equipment for the use of MDRC members located at different sites around the UM medical campus. While CRISPR/Cas9 technology has dramatically increased the speed and decreased the cost at which such models can be generated, the pace at which this new technology continues to evolve prevents many diabetes researchers from taking full advantage of its potential. The MGC fills this gap by using its expertise and personnel to design and construct CRISPR/Cas9 targeting reagents, collaborate with the UM Transgenic Core to test these reagents in embryos and produce founder mice, and identify founders for transfer (along with genotyping protocols) to the MDRC investigator. For the generation of viral reagents, the MGC designs and produces any necessary constructs, which are packaged into viruses by the UM Viral Vector Core. With input from MDRC members and the MGC advisory committee, the MGC also identifies and develops new technologies (viral and genetic) in support of the research programs of MDRC members.