Analytical Cytometry Core

分析细胞计数核心共享资源 摘要 为了提高我们对癌症病因学的理解，并开发出更好的治疗方法， 对于研究人员来说，有能力从复杂的细胞中常规分析和分离离散的细胞类型， 异质种群分析细胞计数核心（ACC）通过以下方式为CC成员履行这一重要职能： 提供基于细胞表面来定义和分离离散免疫系统细胞和癌细胞的服务 标记，以定量和分离表达荧光标记的细胞，并检查离散细胞生长 states. ACC提供流式细胞仪分析和分选仪器（10台分析仪，4台分选仪）， City of Hope Comprehensive Cancer Center（COHCCC）的研究目标。行政协调会维持若干 分析仪器（BC Gallios、BC CyAn、BD C6和两台BD Fortessas）， COH主校区Furth大楼的参数检测。这些仪器可以操作， 由研究人员或核心工作人员。小型颗粒分析仪（例如，外泌体和纳米颗粒; NanoSight NS 300）是 也可以在这个位置。其他分析仪可在整个主校区和城市的 希望生物医学研究中心（BC Gallios，两个BD C6，BD Fortessa和BD Fortessa X-20）。三 高参数细胞分选仪（BD Aria分选仪），位于主校区和City of Hope 生物医学研究中心，由核心员工维护和运营。一个额外的简单的细胞分选仪（生物， RadS3）位于主校区，可供研究人员个人操作。若干附加 仪器已订购，计划于2017年7月交付：BD Fortessa X-20，BD Aria Fusion分拣机，以及 最先进的高维细胞仪（BD FACSymphony）。核心由杰里米·斯塔克博士指导， 癌症遗传学和表观遗传学系教授，并得到高素质工作人员的支持， 维护和操作设备，同时还为希望操作的COHCCC研究人员提供培训 ACC仪器独立。经过培训和授权的用户可以全天候访问仪器， 核心工作人员的时间表是错开的，以便延长核心辅助操作的工作日。监督是 由跨学科的教师咨询委员会提供，并通过年度调查用户反馈。超过 在过去的五年中，ACC被142名独立调查员使用，其中包括94名CC成员。关于94 CC 79名成员（84%）获得了同行审查资金。使用包括来自所有五个程序的成员。因此，ACC 提供方便，具有成本效益和高质量的流式细胞术服务，以支持研究 COHCCC。

Analytical Cytometry Core Shared Resource ABSTRACT To advance our understanding of cancer etiology and to develop improved therapeutic approaches, it is critical for researchers to have the ability to routinely analyze and isolate discrete cell types from complex heterogeneous populations. The Analytical Cytometry Core (ACC) fulfills this vital function for CC members by providing services to define and isolate discrete immune system cells and cancer cells based on cell surface markers, to quantify and isolate cells that express fluorescent markers, and to examine discrete cell growth states. The ACC provides flow cytometry analysis and sorting instrumentation (ten analyzers, four sorters) to support the research goals of the City of Hope Comprehensive Cancer Center (COHCCC). The ACC maintains several analysis instruments (BC Gallios, BC CyAn, BD C6, and two BD Fortessas) that enable varying degrees of parameter detection in the Furth building on the COH main campus. These instruments can be operated either by researchers or by core staff. A small particle analyzer (e.g., exosome and nanoparticle; NanoSight NS300) is also available at this location. Additional analyzers are available throughout the main campus and the City of Hope Biomedical Research Center (BC Gallios, two BD C6, BD Fortessa, and BD Fortessa X-20). Three high-parameter cell sorting instruments (BD Aria sorters), located on the main campus and the City of Hope Biomedical Research Center, are maintained and operated by core staff. An additional simple cell sorter (Bio- Rad S3), located on the main campus, is available for operation by individual researchers. Several additional instruments are on order with scheduled delivery in July 2017: a BD Fortessa X-20, a BD Aria Fusion sorter, and a state-of-the-art high-dimensional cytometer (BD FACSymphony). The core is directed by Dr. Jeremy Stark, a Professor in the Department of Cancer Genetics and Epigenetics, and supported by highly qualified staff that maintain and operate the equipment while also providing training to COHCCC researchers who wish to operate ACC instruments independently. Trained and authorized users have 24/7 access to the instrumentation, and core staff schedules are staggered to allow for an extended workday for core-assisted operation. Oversight is provided by an interdisciplinary faculty Advisory Committee, and user feedback through an annual survey. Over the past five years, the ACC was used by 142 unique investigators, including 94 CC members. Of the 94 CC members, 79 (84%) had peer-reviewed funding. Usage included members from all five Programs. Thus, the ACC provides accessible, cost-effective, and high quality flow cytometry services to support the research of the COHCCC.