Core 3: The Affinity Reagent Characterization Core

摘要 亲和试剂对几乎所有现代人类疾病的科学研究都是必不可少的。抗体是 最广泛使用的亲和试剂家族，当克隆并在培养中生产时，代表着一种强大的 可持续再生的工具。许多单抗相互作用强，脱落率低， 以及良好的特异性。为了补充单抗，加州大学贝克实验室 华盛顿已经开发出技术来设计结合感兴趣分子的小蛋白从头开始。这个 这个名为“亲和力摄政表征核心”的核心的总体目标是提供所需的试剂 项目2和3通过(1)开发用于不同分析的新的单抗，(2)标记 用于细胞和脑成像的抗体和新型小蛋白亲和试剂，以及(3)仔细 对每种试剂进行表征，以确保不同批次的亲和试剂具有相似的行为。

Abstract Affinity reagents are essential to almost any modern scientific investigation of human disease. Antibodies are the most widely used family of affinity reagents and when cloned and produced in culture represent a powerful tool that can be continually regenerated. Many monoclonal antibodies boast strong interactions, slow off-rates, and good specificity. To supplement monoclonal antibodies, the Baker laboratory at the University of Washington has developed techniques to design small proteins de novo that bind molecules of interest. The overall goal of this Core entitled, “Affinity Regent Characterization Core,” is to provide the reagents needed for Projects 2 and 3 by (1) developing new monoclonal antibodies to be used in different assays, (2) labeling antibodies and novel small protein affinity reagents for use in CyTOF and brain imaging, and (3) carefully characterizing each reagent to ensure that different batches of affinity reagents behave similarly.