Physiology Core

摘要 生理学核心的主要目标是为DRC成员提供集中设施， 服务和技术专长，以解决复杂的代谢问题，糖尿病使用正常， 糖尿病或转基因啮齿动物模型。核心结构分为两个子核心，动物外科 和实验程序子核心和分析子核心，每个子核心都包含专门的 设备和关键人员，以帮助刚果民主共和国调查人员和/或其受训人员在最大程度上完成任务 高效和具有成本效益的方式。它也是不同成员之间合作的论坛。 研究背景，但在研究糖尿病的共同兴趣。通过动物外科和 实验程序次核心，DRC研究人员获得培训、设备和实验室设施 和技术专长，进行复杂的啮齿动物手术，包括血管导管的放置， 其他植入物，如脑微注射和微透析探针。核心员工帮助开展复杂的 使用专门的实验方法进行代谢研究（例如葡萄糖钳、示踪剂、微透析 和电流分析研究）--研究人员不容易掌握的技能 没有受过训练或经验的人，特别是年轻的研究人员、研究员和飞行员奖获得者。 分析子核心为DRC成员提供了一个中央设施， 糖调节激素，细胞因子和神经递质，特别是来自啮齿动物的研究。这 生理学核心的组成部分受益于一个持续和多产的专业知识和设备， 放射免疫测定和HPLC设施。扩展基于质谱的测定的所有组成部分， 细胞因子和神经递质DRC -质谱共享资源的建立 并提供最先进的蛋白质检测和翻译后修饰的蛋白质组学方法 量化将有助于大大提高这一核心对刚果民主共和国调查界的价值。在一起， 这两个子核心为刚果民主共和国成员提供了系统地解决有关问题的独特机会 体内机制问题，并评估中枢神经系统和 以最有效和最经济的方式治疗外周组织。

Abstract The primary objective of the Physiology Core is to provide DRC members with access to centralized facilities, services and technical expertise to address complex metabolic questions related to diabetes using normal, diabetic or genetically modified rodent models. The core is structured into two Sub-cores, the Animal Surgery and Experimental Procedure Sub-core and the Analytical Sub-core, each of which contains specialized equipment and key personnel to help DRC investigators and/or their trainees achieve their tasks in the most efficient and cost-effective manner. It also serves as a forum for collaboration between members with different research backgrounds, but a common interest in studying diabetes. Through the Animal Surgery and Experimental Procedure Sub-core, DRC investigators gain access to training, equipment, laboratory facilities and technical expertise to perform complex rodent surgeries, including the placement of vascular catheters and other implantables, such as brain micro-injection and micro-dialysis probes. Core staff help carry out complex metabolic studies using specialized experimental methodologies (e.g. glucose clamps, tracers, microdialysis and amperometric studies) in conscious mice and rats – skills that are not easily accessible to investigators without previous training or experience, particularly young investigators, fellows, and pilot award recipients. The Analytical Sub-core provides DRC members with a central facility for the measurement of glucoregulatory hormones, cytokines and neurotransmitters specifically derived from rodent studies. This component of the Physiology Core benefits from the expertise and equipment of an on-going and prolific radioimmunoassay and HPLC facility. Expansion of the repertoire of mass spectrometry-based assays and establishment of a DRC - Mass Spectrometry Shared Resource to measure cytokines and neurotransmitters and to offer state-of-the-art proteomic methods of protein detection and post-translational modification quantification will serve to greatly enhance the value of this core to the DRC investigator community. Together, these two sub-cores provide DRC members with the unique opportunity to systematically address pertinent mechanistic questions in vivo and to assess metabolic changes in both the central nervous system and peripheral tissues in the most efficient and economical manner.