Diversity Supplement to Elucidating the Orchestrated Bacterial Response to Copper Toxicity

项目摘要 铜对细菌具有广泛的毒性，尽管它们自古以来就与细菌接触。可以看到铜的毒性 在铜表面和工具显著减少医院感染和宿主期间的实践中 介导的营养免疫(隔离必需金属，同时用有毒金属轰击细菌)。 虽然铜中毒的机制包括芬顿化学和混合金属作用，但研究有限。 关于这些机制的研究是有限的。细菌有一个铜输出系统，由一个抑制子组成，一个 铜伴侣和一家铜出口商。所附的R35本多样性补充提案探讨了 细菌，特别是肺炎链球菌是如何通过一种效应和一种作用进化出与铜相互作用的 回应的观点。桑切斯-罗萨里奥女士加入了我的实验室，将致力于澄清这些铜- 肺炎链球菌对细菌反应的特殊机制。

Project Summary Copper is broadly toxic to bacteria despite being them being in contact since antiquity. Copper toxicity is seen in practice where copper surfaces and tools significantly reduce nosocomial infections and during host mediated nutritional immunity (sequestering essential metals while bombarding bacterial with toxic metals). While mechanisms of copper toxicity include Fenton chemistry and mismetallation, research is limited regarding these mechanisms are limited. Bacteria have a copper export system consisting of a repressor, a copper chaperone, and a copper exporter. The R35 this diversity supplement proposal is attached explores how bacteria, specifically Streptococcus pneumoniae evolved to interact with copper both from an effect and response point of view. Ms. Sanchez-Rosario has joined my laboratory and will work to elucidate these copper- specific mechanisms in S. pneumoniae on the side of bacterial responses.