Acquisition of HyPix-6000HE Hybrid Photon Counting Detector and Universal Kappa 4-Axis Goniometer for the X-Ray diffraction system

项目摘要/摘要 在Joshua Sakon教授(Pi X-ray晶体学核心)的实验室中的X-射线衍射系统具有 在全州范围内的结构确定研究工作中发挥并将继续发挥关键作用 生物分子。他从2001年开始参与阿肯色州INBRE，当时阿肯色州布林队是第一个 资金来自国家研究资源中心的拨款。萨肯博士共同执导了 生物技术核心，并在这一角色帮助阿肯色州INBRE网络教职员工和学生访问阿肯色州大学 阿肯色州的核心设施。这笔预算用于购买HyPix-6000HE混合光子计数 用于X射线衍射系统的探测器和万能Kappa四轴测角仪。仪器将会 增强用户对新技术的访问，同时更换不再适用的过时设备 制造商。随着新的安装，土星-92和AFC9的卸载，铍窗的左 端口也将是必需的。该系统于2003年购买，提供了出色的回报 投资了17年。在该设施中解决的81个结构经过验证，并可从 蛋白质数据库(PDB)。该设施提供了我们州存放的78.4%的三维结构 阿肯色州。在2003年−2021年期间，坐标经常被下载(1,685,267次)和 使用PDB实施的网站工具进行检查(503,322次)，并在影响方面领先于我们州(87.9% 下载和查看)。这些结构有助于理解蛋白质的结构和设计新的 分子。该系统的探测器/测角器部分亟需升级。然而，旧的 制造商不再支持更换现有的探测器/测角仪部分 该系统将延长X射线衍射系统的使用寿命，并增加功能和可用性 这个系统。新一代X射线探测器将产生更高质量的数据和软件 与探测器/测角仪一起提供的替换将利用最近开发的先进新算法 即使在相同的信噪比下，也将能够提取更准确的信息。这个 投资将扩大仪器的能力，使其从一个专门用于蛋白质结晶学的系统 到一种能够为小分子产生高质量分析的设备。通过建议的升级 与新的小分子结晶学系统相比，只需更少的资金，该设施就可以继续 满足INBRE、Cobre和NIH支持的该园区调查人员的需求，并 横跨全州。

PROJECT SUMMARY/ ABSTRACT The X-ray diffraction system, in the laboratory of Prof. Joshua Sakon (PI X-ray crystallography core), has played and will continue to play a key role in the statewide research efforts in the structure determination of biomolecules. His involvement in the Arkansas INBRE began in 2001 when the Arkansas BRIN was first funded through a grant from the National Center for Research Resources. Dr. Sakon co-directs the Biotechnology Core and in that role helps Arkansas INBRE network faculty and students access University of Arkansas core facilities. This budget is for the acquisition of the HyPix-6000HE Hybrid Photon Counting Detector and Universal Kappa 4-Axis Goniometer for the X-Ray diffraction system. The instrumentation will enhance user access to new technology while replacing outdated equipment that is no longer serviceable by the manufacturer. With the new installation, the deinstallation of Saturn-92 and AFC9, Beryllium window of left port will also be required. The system, which was purchased in 2003, has provided an excellent return on investment for 17 years. Eighty-one structures solved in the facility are validated and made accessible from the Protein Data Bank (PDB). The facility provided 78.4% of three-dimensional structures deposited from our state of Arkansas. During the period 2003−2021, the coordinates were frequently downloaded (1,685,267 times) and examined using the website tools implemented by PDB (503,322 times) and led our state in impact (87.9% of downloads and views). These structures are aiding in understanding protein architectures and designing new molecules. The detector/goniometer portion of the system is in desperate need of upgrade. However, the old detector is no longer supported by the manufacturer Replacing the existing detector/goniometer portion of the system will extend the life of the X-ray diffraction system as well as increase the functionality and usability of the system. The new generation X-ray detector will produce higher quality data and the software that now comes with the detector/goniometer replacement will leverage advanced new algorithms developed in recent years and will be capable of extracting more accurate information even with the same signal to noise ratio. The investment will extend the capacity of the instrument from being a system dedicated to protein crystallography to one that is capable of also generating high-quality analytics for small molecules. With the proposed upgrade of the system, for less money than a new small molecule crystallography system, the facility can continue to serve the needs of INBRE-, COBRE-, and as well as other NIH-supported investigators on this campus and across the state.