Genomics

项目概要/摘要：基因组学共享资源（GSR） 基因组学共享资源（GSR）的目标是提供先进的基因组学技术和服务 需要支持亚利桑那大学癌症中心进行的全方位癌症研究 （UACC）。单基因到全基因组分析是癌症生物学研究和发现的基石之一。 GSR为UACC成员提供了有指导的、高质量的、具有成本效益的访问全方位的 基因组基石技术。GSR提供以下服务：1）下一代测序; 2） 表观遗传分析; 3）基因表达谱分析; 4）全基因组DNA分析;和5）基因组DNA 方差在这些服务类别中，技术包括全基因组表达谱分析， 比较基因组杂交，基于序列的拷贝数变异，全外显子组测序， 鸟枪测序，靶向测序板，RNA-seq，染色质免疫沉淀（ChIP）-seq， 基因分型、表观基因组ChIP芯片检测、实时定量PCR（qPCR）、样本质量 保证/质量控制（QA/QC）、样品分离、实验咨询、数据分析和数据 存档。GSR具有分析单基因/小基因集以及全基因组分析的能力， 在分析方法上具有很大的灵活性。此外，GSR还提供了开发 定制应用程序，并提供新技术来满足UACC的特定需求 investigators. GSR设备包括：两台Ion Torrent个人基因组机器，一台Ion Torrent质子 (for小到大的下一代测序需求），Affyoung和Agilent微阵列平台（用于 基于杂交的服务）和三台Applied Biosystems qPCR机器（用于全方位服务和自助服务 PCR应用）。GSR提供有关实验设计和样品制备的咨询， 样品QA/QC准备分析。数据分析和存储对于研究者的能力至关重要， 访问他们的数据以便出版和传播。因此，GSR提供了微阵列、序列和生物信息学。 qPCR分析，以支持出版物和赠款的准备。此外，该处还提供 通过密码保护的网站访问原始数据，沿着研究者数据的三重冗余备份， 五年

Project Summary / Abstract: Genomics Shared Resource (GSR) The goal of the Genomics Shared Resource (GSR) is to provide advanced genomics technology and services required to support the full range of cancer research conducted at the University of Arizona Cancer Center (UACC). Single gene to full genome analyses is one cornerstone of cancer biology research and discovery. The GSR provides UACC Members with guided, high-quality, cost-efficient access to a full spectrum of genomic cornerstone technologies. The GSR delivers the following services: 1) next-generation sequencing; 2) epigenetic analysis; 3) gene expression profiling; 4) genome-wide DNA analysis; and 5) genomic DNA variance. Within these categories of service, techniques include whole genome expression profiling, comparative genomic hybridization, sequence-based copy number variance, whole exome sequencing, shotgun sequencing, targeted sequencing panels, RNA-seq, chromatin immunoprecipitation (ChIP)-seq, genotyping, epigenomic ChIP-chip assays, real-time quantitative PCR (qPCR), sample quality assurance/quality control (QA / QC), sample isolation, experimental consultation, data analysis, and data archiving. With the ability to analyze single gene/small gene sets as well as genome wide analyses, the GSR offers great flexibility in analytical approaches. In addition, the GSR provides the capability for developing custom applications and makes new technologies available to address the specific needs of UACC investigators. GSR equipment includes: two Ion Torrent Personal Genome Machines, an Ion Torrent Proton (for small to large next-generation sequencing needs), Affymetrix and Agilent microarray platforms (for hybridization-based services), and three Applied Biosystems qPCR machines (for full service and self-service PCR applications). The GSR provides consultation on experiment design, and sample preparation, followed by sample QA / QC in preparation for the analysis. Data analysis and storage are critical to investigator's ability to access their data for publication and dissemination. Accordingly, the GSR provides microarray, sequence, and qPCR analysis to support the preparation of publications and grants. In addition, the Service provides access to raw data through a password protected website, along with triply redundant backup of investigator's data for five years.