Cleared Tissue Large FOV Microscope Request

此申请申请资金购买米尔滕尼超微火焰荧光光片 显微镜，将包括在现有的集成细胞成像核心(ICIC)。ICIC的首要目标是 是为埃默里和佐治亚州周边机构的调查人员提供尖端的基于光的 显微镜技术、服务和专业知识。这一开放的共享资源已经帮助207项NIH赠款 在过去的5个财年中，142名调查人员。在该设施内，超微火焰将被用于研究 开放和广泛的应用，弥合了从分子水平的荧光专一性到系统的差距 级别分布和连通性。组织清除和光片成像技术提供了高3D 分辨率(~1微米)跨大至10厘米的样品。从分子水平看神经系统中激素的分布 研究跨越整个多刺小鼠大脑半球(凯利)的区域，到转移部位和它们的 全鼠肿瘤学(Lesinski)中相应的肿瘤微环境，这是一种相对较新的显微镜 技术提供了比以往任何时候都更完整的图景。进一步的应用包括3D打印和生物 直接临床应用于心脏病和脑发育的捏造研究(Serposhan)， 黄斑视网膜色素上皮细胞和神经感觉连通性的眼科研究 肝损伤中肝内胆管的3D重建(GRACZ)，以及 人体肠道单细胞核糖核酸转录的三维病理学。这些案例研究 代表埃默里校园巨大兴趣的一小部分，并突出多样性和协作性 机构之间的自然。埃默里/佐治亚州立大学的合作案例研究真正证明了 这种薄片显微镜可以从长度刻度获得广泛的影响，详细说明了如何新颖 分子水平转移调节剂可用于MRI和荧光片成像，提供 对肝转移的关键决定因素(杨，GSU)的独特、全面和纵向的看法。这个 Miltenyi Blaze结合了易用性、灵活性、速度的独特平衡，以及 样本，从而为研究人员提供与疾病相关的纵向问题的快速答案。这 显微镜将作为设施的一部分，按照同样严格的政策进行管理，以确保最佳使用 并最大限度地减少停机时间。埃默里的共享资源具有强大的承诺，其标志是最大的集合 埃默里校区的核心设施套房，位于即将完工的第二健康科学研究中心 大楼(HSRB II)，ICIC有来自医学院和Winship的强大资金承诺 癌症研究所，包括赤字弥补和支持购买资金。我们觉得米尔特尼大火是一场 用于满足NIH资助的研究界当前和未来研究需求的基本工具 在埃默里和周围的机构。

This application requests funds to purchase a Miltenyi UltraMicroscope Blaze fluorescence light sheet microscope, to be included in the existing Integrated Cellular Imaging Core (ICIC). The primary goal of the ICIC is to provide investigators at Emory and surrounding Georgia institutions access to cutting edge light-based microscope technology, services, and expertise. This open shared resource has assisted 207 NIH grants held by 142 investigators in the last 5 FYs. Within the facility, the UltraMicroscope Blaze will be used to study an open and broad range of applications that bridge the gap from molecular level fluorescence specificity to systems level distributions and connectivity. Tissue clearing and light sheet imaging technology provide a high 3D resolution (~1 µm) across samples as large as 10 cm. From molecular level hormone distributions in neurological studies that span regions of an entire spiny mouse brain hemisphere (Kelly), to metastasis sites and their corresponding tumor microenvironments in whole mouse oncology (Lesinski), this relatively new microscopy technique provides a more complete picture than ever before. Further applications include 3D printing and bio fabrication investigations with direct clinical application for heart disease and brain development (Serpooshan), ophthalmological studies of retinal pigment epithelium cells and neurosensory connectivity in macular degeneration (Nickerson), 3D remodeling of intrahepatic bile ducts in liver injury (Gracz), and a combination of 3D pathology with single cell RNA transcriptomics in human intestine (Kugathasan). These case studies represent a small subset of the vast interest on Emory campus, and highlight the diversity and collaborative nature between institutions. An Emory/Georgia State University collaborating case study truly exemplifies the broad impact attainable from length scales encompassed by this light sheet microscope, detailing how novel molecular level metastatic regulators can be utilized in both MRI and fluorescence light sheet imaging, providing a unique, comprehensive, and longitudinal view of the key determinants of liver metastasis (Yang, GSU). The Miltenyi Blaze combines a unique balance of ease of use, flexibility, speed, together with the largest viable samples, thus providing researchers with expedited answers to their disease related longitudinal questions. This microscope will be managed as part of the facilities under the same stringent policies to ensure optimal usage and minimize downtime. The shared resources at Emory have a strong commitment marked by the largest set of core facility suites on Emory campus, housed in the soon to be completed 2nd Health Science Research Building (HSRB II), and the ICIC has a strong financial commitment from the School of Medicine and Winship Cancer Institute, including deficit coverage and supporting purchase funds. We feel the Miltenyi Blaze is an essential tool for addressing both current and future research needs of a largely NIH funded research community at Emory and surrounding institutions.