Two-photon laser-scanning microscope for interdisciplinary collaborations at Weill Cornell

项目摘要 这是神经生理学、神经发育、免疫学的研究人员提出的建议， 微生物学和神经退行性变的共享仪器赠款购买 奥林巴斯透视多光子激发共振扫描(FVMPE-RS)显微镜 配备两台光谱物理钛宝石超快激光器。奥林巴斯FVMPE-RS是一款 创新的最先进的双光子(2P)激光扫描显微镜，允许 两个不同波长的荧光团同时激发和成像，特别是 专为神经科学的活体成像应用而设计。通过允许并发 两种具有可分辨发射光谱的基因编码钙传感器的激发 在不同的神经元亚型中独立表达，有可能评估其激活 以及动物行为中不同神经元网络的相互作用。特别设计的 龙门系统允许显微镜物镜和显微镜之间有355 mm的间隙 底板，使用户可以为特别复杂的用户安装定制的行为设备 与活体成像同时进行的行为分析。同样的设备将 还允许同时进行2P成像和2P光遗传刺激，从而使 研究人员不仅要从神经元网络中记录，而且要操纵他们在 实时，单细胞精度。所要求的仪器将使威尔·康奈尔 调查人员将进行跨越神经生理学、发育的多学科实验 神经生物学、免疫学和微生物学。

Project Summary This is a proposal by investigators in neurophysiology, neurodevelopment, immunology, microbiology, and neurodegeneration for a Shared Instrumentation Grant to purchase an Olympus Fluoview Multiphoton Excitation Resonance Scanning (FVMPE-RS) Microscope equipped with two Spectra-Physics Ti:Sapphire Ultrafast Lasers. The Olympus FVMPE-RS is an innovative, state-of-the-art two-photon (2P) laser-scanning microscope that allows for simultaneous fluorophore excitation and imaging at two different wavelengths and is specially designed for in vivo imaging applications in neuroscience. By permitting the concurrent excitation of two genetically encoded calcium sensors with discernible emission spectra expressed independently in different neuronal subtypes, it is possible to assess the activation and interaction of different neuronal networks in behaving animals. The specially designed gantry system allows for 355 mm of clearance between the microscope objective and the baseplate, enabling users to install customized behavioral equipment for especially complex behavioral assays to be performed concurrently with in vivo imaging. The same equipment will also allow for simultaneous 2P imaging and 2P optogenetic stimulation, enabling the investigators not only to record from neuronal networks but also to manipulate their activity in real time, with single-cell precision. The requested instrument would enable Weill Cornell investigators to conduct multidisciplinary experiments spanning neurophysiology, developmental neurobiology, immunology, and microbiology.