Dissemination of MAPseq and BARseq for high-throughput brain mapping

该项目的目标是将MAPseq和BARseq传播给更广泛的神经科学 社区。这些都是我的实验室基于高通量DNA开发的新方法 用于确定神经元回路的测序。神经元将信息传递给遥远的大脑 通过长距离轴突投射的区域。在某些情况下，功能上不同的种群 神经元在一个很小的区域内混合在一起。连接中断可能是许多 包括自闭症和精神分裂症在内的神经精神障碍。 目前，神经解剖学技术--特别是那些具有单个神经元分辨率的技术--是 价格昂贵，劳动密集型。MAPseq和BARseq将神经解剖学转化为 DNA测序，从而允许我们利用下一步的巨大突破- 生成序列吞吐量。这些高通量方法的传播 确定神经元投射将对理解正常具有重要意义 神经回路，以及在神经精神病学动物模型中这种回路是如何被破坏的 自闭症和精神分裂症等疾病。

The goal of this project is to disseminate MAPseq and BARseq to the broader neuroscience community. These are novel methods developed in my laboratory based on high-throughput DNA sequencing for determining neuronal circuitry. Neurons transmit information to distant brain regions via long-range axonal projections. In some cases, functionally distinct populations of neurons are intermingled within a small region. Disruptions of connectivity may underlie many neuropsychiatric disorders including autism and schizophrenia. At present, neuroanatomical techniques—particularly those with single neuron resolution—are expensive and labor intensive. MAPseq and BARseq convert neuroanatomy into a problem of DNA sequencing, and thereby allow us to exploit the tremendous breakthroughs in next- generation sequencing throughput. The dissemination of these high-throughput methods for determining neuronal projections will have important implications for understanding normal neuronal circuitry, and how this circuitry is disrupted in animal models of neuropsychiatric disorders like autism and schizophrenia.