Cell-type-specific control of information flow between brain regions

摘要/摘要，项目5 这个项目的目标是，在单神经元分辨率下，全面检查 在决策任务中通过大脑传递信息。利用这些专业知识， 从国际脑实验室成员那里获得的知识和数据 并对这一任务进行了研究，提出的方法结合了新颖、先进的技术来实现高性能的数据挖掘。 吞吐量、高分辨率生理记录和解剖示踪以确定 区域间交流的基础。鉴于广泛的功能和结构 大脑中神经元的多样性(例如，听觉皮质中的神经元至少可以投射到 11个不同的目标，形成264个不同的投影图案)，拟议的实验测试 核心假设认为，特定的特权亚群在信息流动中起中介作用 区域以依赖于国家的方式。这样的子群体将根据以下条件进行识别 转录定义的细胞亚型(目标1)和投射类型或基序(目标2)。在活体内二- 光子钙成像将与转录和解剖的体外图谱相结合 通过新的条形码解剖通过测序(BARseq)技术进行身份识别。巴塞克是 用于识别数千或数万的投影和/或遗传标记 一种动物的神经元同时存在。因为这两种技术都是基于 成像，体内数据将与体外数据配准，以支持结构-功能，状态- 在单个神经元水平上的依赖洞察力。这些目标区域和电路 实验将由项目1和2的结果通知。反过来，这里生成的结果 在项目5中将通知项目3中提议的建模工作。

Summary/Abstract, Project 5 The goal of this project is to comprehensively examine, at single-neuron resolution, the flow of information through the brain during a decision-making task. Leveraging the expertise, knowledge, and data gained from International Brain Laboratory members who have designed and studied this task, the proposed approach combines novel, advanced techniques for high- throughput, high-resolution physiological recordings and anatomical tracing to determine the substrates of inter-regional communication. In light of the extensive functional and structural diversity of neurons in the brain (for example, neurons in the auditory cortex can project to at least 11 different targets, forming 264 distinct projection patterns), the proposed experiments test the central hypothesis that specific privileged subpopulations mediate the flow of information between regions in a state-dependent manner. Such subpopulations will be identified based on transcriptionally defined cell subtypes (Aim 1) and projection types or motifs (Aim 2). In vivo two- photon calcium imaging will be combined with in vitro profiling of transcriptional and anatomical identity via the novel barcoded anatomy resolved by sequencing (BARseq) technique. BARseq is used to identify the projections and/or genetic markers of thousands or tens of thousands of neurons simultaneously in a single animal. Because the two techniques are both based on imaging, the in vivo data will be registered with in vitro data to support structure-function, state- dependent insights at the single-neuron level. The regions and circuits targeted in these experiments will be informed by the results of Projects 1 and 2. In turn, the results generated here in Project 5 will inform the modeling work proposed in Project 3.