Role of periostin expressing cells in intramembranous bone regeneration

项目摘要/摘要：该提案包括一项为期5年的全面指导职业发展计划 计划让Frank Ko博士转变为研究膜内骨的独立研究员 再生。这位候选人目前是细胞和分子医学系的助理教授 (CMM)在拉什大学医学中心(RUMC)，并在RUMC及其附属机构进行研究 中心和机构。他得到了研究所的大力支持，将把100%的时间投入到他的研究中 和职业发展计划。这位候选人已经召集了导师和一个科学咨询委员会， 广泛的互补专业知识和丰富的指导经验，在 他过渡到独立研究，并为他的计划培训新的智力和技术技能 学习。他的导师里克·萨姆纳博士是骨再生和种植体固定领域的专家， RUMC的CMM主席，以及Rush MicroCT/组织学核心设施的主任。他的合作导师，安娜博士 Spagnoli是一位临床医生兼科学家，在骨骼发育和疾病领域拥有专业知识，是 RUMC整形外科分子医学科主任。科学 顾问委员会成员小野洋明博士是研究骨骼干细胞和祖细胞的分子生物学家。 细胞。这门科学的重点是Periostin表达细胞在膜内骨再生中的作用 在骨髓消融手术后。这位候选人发现，这些细胞通常不存在于 骨髓和驻留在骨膜中，存在于手术后再生的骨髓腔中 它们的耗尽延缓了膜内骨的再生。因此，具体目的是调查：1) 膜内骨再生中Periostin表达细胞和Periostin的必要性；2)谱系 Periostin表达细胞及其后代的承诺、分子特征和分化 膜内骨再生。补充他之前在骨生物力学和分子方面的训练 在完整组织中的生物学方面，应聘者将接受使用报告鼠、动物外科和 再生骨表型，包括光片和扩增显微镜、基因和蛋白表达 分析，以及体外培养和检测。此外，他将参加职业发展活动， 包括组织再生、干细胞生物学、生物统计学 授予写作、负责任的研究行为和科学陈述。这份K01提案将允许 候选人将获得宝贵的职业发展指导，以实现成为独立人士的目标 研究膜内骨再生的调查者。

Project Summary/Abstract: The proposal includes a comprehensive 5-year mentored career development plan for Dr. Frank Ko to transition to an independent investigator who studies intramembranous bone regeneration. The candidate is currently an assistant professor in the Department of Cell & Molecular Medicine (CMM) at Rush University Medical Center (RUMC) and conducts his research at RUMC and at its affiliated centers and institutions. He is well supported by the institution and will devote 100% of his time to his research and career development plan. The candidate has assembled mentors and a scientific advisory committee with a wide array of complementary expertise and extensive mentoring experience to offer career guidance during his transition to independent research and train him in new intellectual and technical skills for his proposed studies. His mentor, Dr. Rick Sumner, is an expert in the field of bone regeneration and implant fixation, the Chair of CMM at RUMC, and the director of Rush MicroCT/Histology Core Facility. His co-mentor, Dr. Anna Spagnoli, is a clinician-scientist with an expertise in the field of skeletal development and diseases and is the director of Section of Molecular Medicine in the Department of Orthopedic Surgery at RUMC. Scientific advisory committee member Dr. Noriaki Ono is a molecular biologist studying skeletal stem and progenitor cells. The science focuses on the role of periostin-expressing cells in intramembranous bone regeneration following marrow ablation surgery. The candidate discovered that these cells, which are normally absent in marrow and reside in the periosteum, are present in the regenerating marrow cavity following the surgery and their depletion delays intramembranous bone regeneration. Thus, the specific aims are to investigate: 1) the necessity of periostin-expressing cells and periostin in intramembranous bone regeneration; and 2) the lineage commitment, molecular signatures, and differentiation of periostin-expressing cells and their progeny during intramembranous bone regeneration. To complement his prior training in bone biomechanics and molecular biology in intact tissues, the candidate will be trained in the use of reporter mice, animal surgery, and regenerated bone phenotyping, including light sheet and expansion microscopy, gene and protein expression analyses, and in vitro culture and assay. Further, he will participate in career development activities that include didactic lectures and seminar series focusing on tissue regeneration, stem cell biology, biostatistics, grant writing, responsible conduct of research, and scientific presentation. This K01 proposal will allow the candidate to gain valuable career development mentoring to achieve his goal of becoming an independent investigator studying intramembranous bone regeneration.