Role of periostin expressing cells in intramembranous bone regeneration

项目摘要/摘要：膜内骨再生在以下几个方面发挥着重要作用 骨科和牙科手术，如种植、骨折愈合和牵张成骨。 先前的研究已经研究了膜内骨再生的不同过程，例如 炎症、血管生成和骨再生。然而，膜内的一个未被研究的方面 骨再生是痛觉神经元的一种，如它们的分布和形态变化 膜内骨再生过程。对疼痛感觉神经元的严格检查可以 通过组织透明和光片显微镜实现，允许三维可视化和 对组织进行定量。然而，实施这些方法遇到了重大的技术问题 在肌肉骨骼组织中的挑战，例如显著的自体荧光和由于 到富含胶原蛋白的细胞外基质。我们的初步数据展示了几种克服 这些挑战，但对传统组织学方法的进一步完善和比较研究是 需要的。因此，我们提出了这种补充剂，旨在开发肌肉骨骼组织特异性 组织透明光片显微镜，将检查Periostin表达细胞和痛觉神经元 在膜内骨再生过程中。补编将涉及战略目标“目标B：扩大”。 风湿、皮肤和肌肉骨骼疾病疼痛研究能力(队列、技术或数据) 目前的研究基地/合作者“在新的疼痛成像技术方面具有特定的科学领域 肌肉骨骼组织。

Project Summary/Abstract: Intramembranous bone regeneration plays an important role in several orthopaedic and dental procedures such as implant placement, fracture healing, and distraction osteogenesis. Prior studies have examined different processes during intramembranous bone regeneration, such as inflammation, angiogenesis, and bone regeneration. However, an under explored aspect of intramembranous bone regeneration is pain sensing neurons, such as their distribution and morphological changes over the course of intramembranous bone regeneration. A rigorous examination of pain sensing neurons can be achieved by tissue clearing and light sheet microscopy, which allows three-dimensional visualization and quantitation of tissues. However, implementing these approaches have been met with significant technical challenges in musculoskeletal tissues, such as significant autofluorescence and insufficient depth of field due to collagen-rich extracellular matrix. Our preliminary data demonstrate several approaches that overcome these challenges, but further refinement and comparison studies to conventional histological approaches are needed. Therefore, we are proposing this supplement that aims to develop musculoskeletal tissue specific tissue clearing light sheet microscopy that will examine periostin expressing cells and pain sensing neurons during intramembranous bone regeneration. The supplement will address the strategic goal “Goal B: Expand rheumatic, skin, and musculoskeletal disease pain research capacity (cohorts, technologies or data) beyond current research base/collaborators” with a specific scientific area in novel pain imaging technologies for musculoskeletal tissues.