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Role of periostin expressing cells in intramembranous bone regeneration

骨膜蛋白表达细胞在膜内骨再生中的作用

基本信息

批准号：
10856528
负责人：
Frank Ko
金额：
$ 6.35万
依托单位：
RUSH UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-01 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10856528
关键词：
Ablation Administrative Supplement Antibodies Area Bone Marrow Ablation Bone Regeneration Bone Tissue Brain Cartilage Cells Collagen Data Dental Care Detergents Distraction Osteogenesis Enzymes Extracellular Matrix Failure Fluorescent Dyes Goals Histologic Image Imaging technology Impairment Implant Implantation procedure Inflammation Knee joint Label Light Limb structure Marrow Microscopy Molecular Morphology Mus Muscle Musculoskeletal Musculoskeletal Diseases Neurons Nociceptors Operative Surgical Procedures Orthopedic Procedures Orthopedics Pain Pain Research Parents Patients Periosteum Permeability Photobleaching Play Process Proteins Refractive Indices Research Rheumatism Role Signal Transduction Skeletal Muscle Solvents Spinal Cord Technology Temporomandibular Joint Tendon structure Therapeutic Time Tissues Visualization angiogenesis antibody conjugate base bone bone fracture repair cohort collagenase imaging approach imaging modality intramembranous bone microscopic imaging novel parent grant periostin preservation skin disorder soft tissue spatiotemporal three-dimensional visualization

项目摘要

Project Summary/Abstract: Intramembranous bone regeneration plays an important role in several orthopaedic and dental procedures such as implant placement, fracture healing, and distraction osteogenesis. Prior studies have examined different processes during intramembranous bone regeneration, such as inflammation, angiogenesis, and bone regeneration. However, an under explored aspect of intramembranous bone regeneration is pain sensing neurons, such as their distribution and morphological changes over the course of intramembranous bone regeneration. A rigorous examination of pain sensing neurons can be achieved by tissue clearing and light sheet microscopy, which allows three-dimensional visualization and quantitation of tissues. However, implementing these approaches have been met with significant technical challenges in musculoskeletal tissues, such as significant autofluorescence and insufficient depth of field due to collagen-rich extracellular matrix. Our preliminary data demonstrate several approaches that overcome these challenges, but further refinement and comparison studies to conventional histological approaches are needed. Therefore, we are proposing this supplement that aims to develop musculoskeletal tissue specific tissue clearing light sheet microscopy that will examine periostin expressing cells and pain sensing neurons during intramembranous bone regeneration. The supplement will address the strategic goal “Goal B: Expand rheumatic, skin, and musculoskeletal disease pain research capacity (cohorts, technologies or data) beyond current research base/collaborators” with a specific scientific area in novel pain imaging technologies for musculoskeletal tissues.

项目摘要/摘要：膜内骨再生在几个方面起着重要作用，整形外科和牙科手术，如植入物放置、骨折愈合和牵引成骨。先前的研究已经检查了膜内骨再生过程中的不同过程，例如炎症、血管生成和骨再生。然而，膜内的一个未被探索的方面，骨再生是痛觉神经元，如它们的分布和形态变化，膜内骨再生过程。对痛觉神经元的严格检查可以通过组织清除和光片显微镜实现，其允许三维可视化，组织的定量。然而，实施这些方法遇到了重大的技术问题。肌肉骨骼组织中的挑战，例如显著的自体荧光和由于富含胶原的细胞外基质我们的初步数据表明，几种方法，克服这些挑战，但进一步完善和比较研究，传统的组织学方法， needed.因此，我们建议这种补充，旨在发展肌肉骨骼组织特异性组织透明光片显微镜将检查骨膜蛋白表达细胞和痛觉神经元在膜内骨再生过程中。补编将涉及战略目标“目标B：扩大风湿性、皮肤和肌肉骨骼疾病疼痛研究能力（队列、技术或数据）目前的研究基地/合作者”，在新的疼痛成像技术的特定科学领域，肌肉骨骼组织

项目成果

期刊论文数量（2）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Activation of canonical Wnt signaling accelerates intramembranous bone regeneration in male mice.

DOI：
10.1002/jor.25217
发表时间：
2022-08
期刊：
JOURNAL OF ORTHOPAEDIC RESEARCH
影响因子：
2.8
作者：
Ko, Frank C.;Moran, Meghan M.;Ross, Ryan D.;Sumner, D. Rick
通讯作者：
Sumner, D. Rick

Colon epithelial cell-specific Bmal1 deletion impairs bone formation in mice.

结肠上皮细胞特异性 Bmal1 缺失会损害小鼠的骨形成。

DOI：
10.1016/j.bone.2022.116650
发表时间：
2023
期刊：
Bone
影响因子：
4.1
作者：
Ko,FrankC;Jochum,SarahB;Wilson,BrittanyM;Adra,Amal;Patel,Nikhil;Lee,Hoomin;Wilber,Sherry;Shaikh,Maliha;Forsyth,Christopher;Keshavarzian,Ali;Swanson,GarthR;Sumner,DRick
通讯作者：
Sumner,DRick