Bone Cells Analysis

拟议的骨细胞分析核心(BCAC)将使参与的调查人员能够 利用最先进的骨细胞技术开展骨骼生物学研究的新方向 分离、功能鉴定和RNA测序。拟议的核心将把 其领导层的专门知识(主任Pajevic Divieti博士和副主任Gardella博士， Gerstenfeld和Warman)和强大的新研究方法和检测系统 将极大地丰富和催化科学进步的协同核心资源 参与调查的人员。 BCAC将为中心调查人员(CI)提供1)临床前和临床骨细胞以及 基因工具，2)细胞信号和生物标记分析服务，以及3)RNA测序服务。 骨细胞核心(波士顿大学的Divieti Pajevic和Gerstenfeld博士)将提供老鼠- 来自野生型或转基因动物的衍生骨细胞和细胞系。核心意志 还与格斯滕菲尔德博士(协理主任)合作，提供高度注释的人 全髋关节置换的标本。BCAC将提供广泛的内部和 信令分析服务下的商业可用信令分析(Gardella博士， 内分泌科副主任)。核心将帮助研究设计，并将培训 研究人员对这些技术的各个方面都很了解。RNA测序核心(沃曼博士， 波士顿儿童医院副主任)将提供服务，帮助CI及其学员 设计、执行和分析批量和单细胞RNA测序实验，包括 骨骼细胞和组织。Core将为设计批量和单细胞RNA提供帮助 帮助进行测序实验，并协助组织和细胞准备以回收RNA。这个 CORE将提供微型粉碎机和优化方法，从骨骼和 软骨用于批量RNA测序，活细胞用于单细胞RNA测序。核心意志 使用专为非常低的起始RNA量设计的试剂盒来生成测序库。《核心》 将协助对测序数据进行信息学分析。 核心提供的每项服务都将严格控制质量、性能和 可靠性。核心将举办实践培训课程，通过这些课程，调查人员可以 指导和教授如何进行分析、细胞操作和RNA测序。 它还将建立一系列研讨会，在研讨会上，新的方法、方法和相关的 本课程将介绍和讨论科学进展。 好了！

The proposed Bone Cells Analysis Core (BCAC) will enable participating investigators to pursue new lines of research in skeletal biology by using state-of-the-art technologies for bone cell isolation, functional assessment, and RNA sequencing. The proposed Core will bring together the expertise of its leadership (Director Dr. Pajevic Divieti and the Associate Directors Drs. Gardella, Gerstenfeld and Warman) and powerful new research approaches and assay systems to make for a synergistic Core Resource that will greatly enrich and catalyze the scientific advances of participating investigators. The BCAC will provide Center investigators (CIs) with 1) Preclinical and clinical bone cells and genetic tools, 2) Cell Signaling and Biomarker Assay services, and 3) RNA sequencing services. The Bone cell Core (Drs Divieti Pajevic and Gerstenfeld, Boston University), will provide mouse- derived bone cells and cells lines from both wild type or genetically modified animals. The Core will also provide, in collaboration with Dr. Gerstenfeld (Associate Director), highly annotated human specimens from total hip replacements. The BCAC will offer a wide array of in-house and commercially available signaling assays under the Signaling Assay service (Dr. Gardella, Associate Director, Endocrine Unit, MGH). The Core will help with the study design and will train investigators in all the aspects of these techniques. The RNA sequencing core (Dr. Warman, Associate Director, Boston Children’s Hospital) will provide services to help CIs and their trainees design, perform, and analyze bulk and single cell RNA sequencing experiments that involve skeletal cells and tissues. The Core will provide help with designing bulk and single cell RNA sequencing experiments and with assisting with tissue and cell preparation for RNA recovery. The core will provide micro-pulverizers and optimized methods for recovering RNA from bone and cartilage for bulk RNA sequencing and living cells for single cell RNA sequencing. The Core will generate sequencing libraries using kits designed for very low amounts of starting RNA. The Core will assist in the informatics analysis of the sequencing data. Each service provided by the Core will be rigorously controlled for quality performance and reliability. The Core will conduct hands-on training sessions by which investigators can be mentored and taught how to perform assays, cell manipulations on their own and RNA sequencing. It will also establish a seminar series in which new methodologies, approaches, and related scientific progress will be presented and discussed. !