Core 3: Mouse GBM models and Imaging Core

核心3摘要 为了确保使用的小鼠胶质母细胞瘤(GBM)模型的重复性、严密性和协调性 项目2、3和4，我们已经建立了一个专门用于该计划的鼠标GBM模型核心 该项目并不存在于参与机构内。核心提供广泛的集中访问 负责维护和测试小鼠GBM肿瘤的中央存储库和专业知识 与其立体定向植入小鼠脑内相关的研究和分析，包括颅内成像 GBMS。它还一直在提供小鼠GBM新抗原的发现和验证。这将包括访问 对实验目标至关重要的小鼠细胞系及其衍生品。这更容易实现 通过核心，而不是通过个人(例如R01)授权机制或通过现有的授权机制 NIH赞助的P01机构的服务核心。这将通过追求两个具体目标来实现： 生成和维护小鼠GBM细胞库，设计表达感兴趣转基因的GBM细胞， 鉴定和验证小鼠GBM新抗原(目标1)，并提供技术专长和性能 用于小鼠脑内GBM的立体定向植入及其系列成像(目标2)。提供以下服务 4个项目中的3个项目的这些服务将减少费用，减少技术和样品偏差，并将确保 这3个项目将使用相同的特征良好的小鼠GBM细胞，这些细胞被植入并进行成像 专业技术人员。

Core 3 Abstract To ensure reproducibility, rigor and harmonization amongst the mouse glioblastoma (GBM) models utilized by Projects 2, 3 and 4, we have established a mouse GBM model Core that is exclusively utilized for this Program Project and does not exist within participating Institutions. The Core provides broad centralized access to a central repository responsible for the maintenance and testing of mouse GBM tumors and professional expertise related to their stereotactic implantation into mouse brains and the analysis, including imaging of intracranial GBMs. It also has been providing mouse GBM neoantigen discovery and validation. This would include access to mouse cell lines and their derivatives critical to the experimental aims. This is more easily accomplished through the Core and not as easily done through individual (e.g. R01) granting mechanisms or through existing NIH sponsored service cores at the P01 institutions. This will be accomplished by pursuing two specific aims: generate and maintain a repository of mouse GBM cells, engineer GBM cells that express transgenes of interest, and identify and validate mouse GBM neoantigens (Aim 1) and provide the technical expertise and performance for the stereotactic implantation of mouse GBMs in brains and for their serial imaging (Aim 2). The provision of these services for 3 of the 4 Projects will reduce expense, will reduce technical and sample bias and will ensure that the 3 Projects will employ the same well characterized mouse GBM cells that are implanted and imaged by expert technical personnel.