Physiology

项目摘要-生理学核心 该视觉研究核心（VRC）的总体目标是提供： ·获得个别R 01奖励范围以外的资源 ·获得单一实验室范围之外的技术专长 ·员工培训，以消除有效转化研究和合作的障碍 ·VRC实验室之间的合作计划 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发展障碍，青光眼和其他疾病，以及一系列尖端的基础科学 举措我们已经实现了四个资源模块，继续自然演变， 研究小组一起工作，为17名研究人员提供服务，持有22个NEI R 01奖项。 生理学模块是VRC教师用于动物模型验证和疾病的一套功能强大的工具 分析：ERG，OptoMotry行为测试和一系列眼部成像工具（OCT，Micron）。我们的新 模块包括体内/体外双光子成像。 具体而言，生理学模块提供： ·三台UTAS ERG系统（两台在JMEC动物园内） ·JMEC动物园内的OptoMotry系统 ·两个Micron小鼠成像系统（一个在JMEC动物园内） ·额外的新型Micron IV鼠标成像系统，具有高分辨率OCT、图像引导ERG 功能，裂隙灯和聚焦激光损伤子模块，最近获得的资金从该部 和NEI的管理补充（P30 EY 014800 - 14 S1） ·JMEC动物园内用于运动和前庭表型分析的旋转棒 ·双Spectralis OCT系统（JMEC动物园内的一个） ·Bruker Ultima双光子显微镜（位于5楼核心） ·为所有这些系统提供技术支持

Project Summary-Physiology Core The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration · collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Physiology Module is a powerful set of tools that VRC faculty use for animal model validation and disease profiling: ERGs, OptoMotry behavioral testing, and a range of ocular imaging tools (OCT, Micron). Our new module includes in vivo / in vitro 2-photon imaging. Specifically the Physiology Module provides: · Three UTAS ERG systems (two within the JMEC vivarium) · An OptoMotry system within the JMEC vivarium · Two Micron mouse imaging systems (one within the JMEC vivarium) · An additional new Micron IV mouse imaging system with high resolution OCT, image-guided ERG function, slit lamp and focal laser injury submodules, recently acquired with funds from the Department of Ophthalmology and an Administrative supplement of the NEI (P30EY014800-14S1) · Rotarod for locomotor and vestibular phenotyping within the JMEC vivarium · Dual Spectralis OCT systems (one within the JMEC vivarium) · A Bruker Ultima 2-photon microscope (in core on floor 5) · Technical support for all these systems