In Vivo Therapeutics Core

摘要：在活体治疗核心(IVTC) 体内治疗核心(IVTC)是印第安纳大学梅尔文分校的一个多方面的共享资源设施 以及布伦·西蒙癌症中心(IUSCC)，该中心的成立是为了促进新的发现和测试 药理和细胞疗法。IVTC分为五个研究专业中心：最先进的 用于癌症研究的设备，专业小鼠品系的培育，临床前毒性和疗效测试 异种移植模型、患者来源的异种移植和造血干细胞移植研究。重要的是， IVTC的组织方式是提供必要的基础设施、监督和所需的专业知识 成功地满足了IUSCC调查人员的需求。鉴于IUSCC调查人员严重依赖于 由IVTC提供的服务，有很强的理由支持这种集中式共享资源，这将促进 印第安纳州集水区癌症新疗法的发现。在过去一年中，67%的 IVTC用户是同行评审的癌症中心成员，占开具发票的服务的94%。为 对于IUSCC的大多数成员来说，利用小鼠模型是其正在进行的研究的重要组成部分 活动的重点是液体和固体肿瘤新疗法的开发。以支持 作为会员，IVTC的目标是：(1)维持现场繁育的小鼠群体并建立 患者来源的异种移植物对于探索、测试和提炼新的癌症是必不可少的 治疗；(2)提供适用于体内研究的专业核心服务和技术；以及 (3)为IUSCC调查人员和受训人员提供咨询和教育资源。具体来说， IVTC管理辐射设施，并将执行体外和体内所需的辐射服务 实验，包括造血干细胞移植。IVTC将协调以下各方面 程序，并在实验设计、法规要求、方法和数据方面提供帮助 为每个研究中心提供口译服务。IVTC维护的共享设备为调查人员提供了 访问最近获得的IncuCyteZOOM活细胞成像系统和低氧工作站，它们是 两者均可直接使用或与IVTC的技术人员合作使用。此外，IVTC将与 IUSCC研究小组协调及时将老鼠和样本送到IUSCC内的其他核心设施 以及IUSM。作为一个集中的设施，IVTC将为IUSCC调查人员提供以下高级资源 对新药靶标和生物标记物的临床前验证至关重要的分析性和调查性研究 因此，可以成功地开发由研究人员发起的创新试验。

ABSTRACT, In Vivo Therapeutics Core (IVTC) The In Vivo Therapeutics Core (IVTC) is a multi-faceted shared resource facility of the Indiana University Melvin and Bren Simon Cancer Center (IUSCC) that was established to facilitate the discovery and testing of novel pharmacological & cellular therapies. The IVTC is divided into five hubs of research expertise: state-of-the-art equipment for cancer research, breeding of specialized mouse strains, preclinical toxicity and efficacy testing in xenograft models, patient-derived xenografts, and hematopoietic stem-cell transplant studies. Importantly, the IVTC is structured in a way to provide the necessary infrastructure, oversight, and expertise required to successfully meet the needs of the IUSCC investigators. Given the heavy reliance of IUSCC investigators on services provided by IVTC, there is strong rationale to support this centralized shared resource that will facilitate the discovery of new therapies for cancers prominent in the catchment area of Indiana. In the past year, 67% of IVTC users were peer-reviewed Cancer Center members and accounted for 94% of the services invoiced. For the majority of IUSCC members, utilization of mouse models is an essential component of their ongoing research activities focused on development of novel therapies for liquid and solid tumors. To support the needs of the membership, the Aims of the IVTC are: (1) to maintain on-site breeding colonies of mice and establish patient-derived xenografts essential for the exploration, testing, and refinement of novel cancer therapeutics; (2) to provide specialized core services and technology applicable to in vivo studies; and (3) to serve as a consultation and educational resource for IUSCC investigators and trainees. Specifically, the IVTC manages the Radiation facility and will perform irradiation services needed for in vitro and in vivo experiments, including hematopoietic stem-cell transplantations. The IVTC will coordinate all aspects of procedures and provide assistance with experimental design, regulatory requirements, methodology, and data interpretation for each research hub. Shared equipment maintained by the IVTC provides investigators with access to a recently acquired IncuCyteZOOM Live-Cell Imaging System and a hypoxia workstation which are both available for direct use or in partnership with technical staff in the IVTC. In addition, the IVTC will work with IUSCC research teams to coordinate timely delivery of mice and samples to other Core facilities within the IUSCC and the IUSM. As a centralized facility, the IVTC will provide IUSCC investigators advanced resources for analytical and investigational studies essential for the preclinical validation of novel drug targets and biomarkers of cancer so that innovative investigator-initiated trials can be successfully developed.