Toxicological screening in a single animal format

摘要 动物毒性试验在成本、时间、可靠性和动物方面都有局限性。 福利。我们建议在单一动物模型中开发一种新的毒理学筛选方法 用于快速评估化学品的安全性，减少动物使用和改良动物 福利。我们将开发一种新的测试程序，使用该程序，我们只需在一个 动物获得关于有效剂量、受影响的组织和器官的信息，以及 毒物的毒理动力学，通常需要在数百只动物身上进行实验，几个月 经过多年的努力和大量的生化和病理分析，在传统的 毒理学研究。这项突破性的技术是一种实时绘制细胞图的报告系统 化学物质对全身造成的损害。它可以检测到的毒性作用 物质早在细胞损伤时就会在体内发生。而在传统的动物试验中， 不良反应只有在表现出显著程度的 在接受大剂量的测试化学品后，对健康的不利影响。而不是带走这只动物 在传统的检测中，身体就像黑匣子一样，我们的新技术使实时监控成为可能 通过使动物身体透明以直接观察有害物质来对化学物质的反应 在细胞水平上的影响。

Abstract Toxicity testing with animals has limitations with regards to cost, time, reliability, and animal welfare. We propose to develop a novel toxicological screening approach in a single animal format for quickly assessing the safety of chemicals with reduction of animal use and improved animal welfare. We will develop a new testing procedure with which we could perform tests in only one animal to obtain information on the effective doses, impacted tissues, and organs, and toxicodynamics of toxicants, which usually need experimenting on hundreds of animals, months to years efforts, and enormous biochemical and pathological analyses in the traditional toxicological study. The breakthrough technique is a reporter system for real-time mapping cellular damage caused by chemical substances in the whole body. It can detect the toxic effects of substances as early as cellular damage occurs in the body. Whereas in traditional animal testing, adverse effects only become detectable/measurable in animals exhibiting significant degrees of adverse health effects after receiving high doses of test chemicals. Instead of taking the animal body as a "black box" in the traditional testing, our new technique enables real-time monitoring responses to a chemical by making the animal body transparent for directly observing adverse effects at the cellular level.