Microwave-Enhanced Microreactor for Ultrafast Detection of Protein Biomarkers

项目摘要 在诊断、风险分层和危及生命的疾病/状况的管理中， 对许多相关蛋白质生物标志物的分钟计数和明确检测至关重要。 不幸的是，目前明确的方法-质谱法-的工作流程是 通过样品制备过程进行检查。一种微波增强的， 本文提出了一种用于蛋白质选择性捕获和消化的微反应器，以解决 这一重大限制。第一阶段STTR的具体目标是创建一个原型 微反应器，并证明了微反应器使能质量的概念证明 蛋白质生物标志物的光谱分析。 拟议的技术和产品将简化质谱分析的工作流程 分析蛋白质，将最终用户从繁重和繁琐的多样品制备中解放出来 步骤，并允许近实时和明确的检测蛋白质生物标志物，以及 治疗剂、药物靶点等。

Project Summary In diagnosis, risk stratification, and management of life-threatening diseases/conditions, every minute counts and unambiguous detection of many relevant protein biomarkers is crucial. Unfortunately, the workflow of current unambiguous approach–mass spectrometry–is bottlenecked by the sample preparation process. A microwave-enhanced, flow-through microreactor is proposed here for both selective capture and digestion of proteins to address this major limitation. The specific aims of this Phase I STTR are to create a prototype microreactor and to demonstrate a proof of concept of the microreactor-enabled mass spectrometry analysis of protein biomarkers. The proposed technology and product will streamline the workflow of mass spectrometry analysis of proteins, free end users from the laborious and tedious multiple sample preparation steps, and allow near real-time and unambiguous detection of protein biomarkers, as well as therapeutics, drug targets, etc.