P30-Core Grant for Vision Research

总体汇总 要求继续支持NEI视觉研究核心补助金，以支持 亚特兰大视觉研究社区（AVRC）。本中心补助金更新申请包括三个 资源/服务核心：（1）结构生物学和成像，（2）功能基因组学和蛋白质组学，以及（3） 生物信息学和生物统计学。AVRC核心补助金支持协作视觉研究和服务 在23名教师中，有18名符合条件的NEI资助的R 01，24名博士后研究员和38名博士前研究员。这 支持主要来自AVRC人员的专业知识和服务，他们对AVRC教职员工的培训， 以及设备、供应品和其他资源的可用性。在过去的五年里，核心赠款为 产生了193篇同行评议的出版物，许多新的和竞争性的更新NEI资助的 R01在未来5年，我们正在积极招聘5-7初级研究终身教职的教师谁将重新- 激励AVRC和EEC，增加P30核心补助金的需求，价值和科学影响。 结构生物学和成像（SBI）核心为AVRC的成员提供资源和服务， 关于确定健康人眼结构的正常和异常结构之间的关系， 组织和疾病模型中有和没有实验疗法。为结构提供支持- 功能研究，重点是视网膜功能、视网膜变性/损伤、RPE形态测量、眼 肿瘤学和基因/药物递送。Core支持超声检查、免疫组化、共聚焦 显微镜、荧光图像分析以及光学和电子显微镜服务。 功能基因组学和蛋白质组学（FG&P）核心的目标是加强研究，提高我们的 了解生物功能（例如，视觉）来自生物体的编码信息。 基因组并通过表观遗传学进行修饰。核心支持分子、生物化学和表型 通过六个部分进行实验：DNA/RNA服务，蛋白质/小分子分析，细胞培养， 眼/视觉结构和功能分析，啮齿动物眼显微手术和小鼠群体服务。 方法包括扫描激光检眼镜，SD-OCT，眼底成像，精密测微， 视网膜电图、OKT、HPLC、LC-MS、PCR、LCM、免疫印迹和ELISA、流式细胞仪。 流式细胞术、蛋白质测序、微阵列分析和NexGen测序。 生物信息学和生物统计学（生物分析）核心提供生物统计学，生物信息学，数据管理， 电子笔记本、数据备份和高级计算分析。支持包括努力 核心专用的信息学家和统计学家、先进的软件和云计算访问。 咨询委员会协助核心董事确保设施得到充分和公平的使用，并就以下事项提供建议： 根据需要创造新的资源或消除未充分利用的组件。核心们 提高并将继续提高研究生产力，提供共同服务， 促进教师之间的合作研究，并吸引其他学科的视觉研究。

Overall Summary Continued support is requested for the NEI Core Grant for Vision Research that supports investigators of the Atlanta Vision Research Community (AVRC). This Center Grant renewal application includes three Resource/Service Cores: (1) Structural Biology and Imaging, (2) Functional Genomics and Proteomics, and (3) Bioinformatics and Biostatistics. The AVRC Core grant supports collaborative vision research and service among 23 faculty with 18 eligible NEI funded R01s, 24 postdoctoral fellows, and 38 predoctoral fellows. This support comes largely via the expertise and service of AVRC personnel, their training of AVRC faculty and staff, and the availability of equipment, supplies, and other resources. In the past 5 years, the Core grant contributed to the generation of 193 peer-reviewed publications and to many new and competing renewal NEI-funded R01s. During the next 5 years, we are actively recruiting 5-7 junior research tenure-track faculty who will re- invigorate the AVRC and the EEC, increasing the need, value, and scientific impact of this P30 Core grant. The Structural Biology and Imaging (SBI) Core provides resources and services for members of the AVRC with regard to determining the relationship between normal and abnormal structure of ocular structures in healthy tissue and in disease models with and without experimental therapies. Support is provided for structure- function studies with an emphasis on retinal function, retinal degeneration/injury, RPE morphometry, ocular oncology and gene/drug delivery. The Core supports ultrasonography, immunohistochemistry, confocal microscopy, fluorescence image analysis, and light and electron microscopy services. The goal of the Functional Genomics and Proteomics (FG&P) Core is to enhance research that increases our understanding of how biological function (e.g., vision) arises from the information encoded in an organism's genome and is modified by epigenetics. The Core supports molecular, biochemical, and phenotypic experiments via six sections: DNA/RNA services, protein/small molecule analysis, cell culture, and ocular/vision structural and functional analysis, rodent ocular microsurgery, and mouse colony services. Approaches include scanning laser ophthalmoscopy, SD-OCT, fundus imaging, precision micrometry, photorefraction, rebound tonometry, ERG, OKT, HPLC, LC-MS, PCR, LCM, immunoblotting and ELISA, flow- cytometry, protein sequencing, microarray analysis, and NexGen sequencing. The Bioinformatics and Biostatistics (Bioanalysis) Core provides biostatistics, bioinformatics, data management, electronic notebooks, data backups, and advanced computational analyses. The support includes effort of Core-dedicated informaticists and statisticians, advanced software, and cloud-computing access. An Advisory Committee aids the Core directors to insure full and equitable use of the facilities and to advise on creation of new resources based on need or elimination of components that are underutilized. The Cores have increased and will continue to increase research productivity by providing common services to stimulate and facilitate collaborative studies among faculty and to attract other academic disciplines to vision research.