Comparative Pathology Core

项目总结-比较病理学核心 比较病理学核心（CPC）是一个既定的共享资源， 宾夕法尼亚大学兽医学院（Penn Vet）的病理生物学，以及一个完全集成的 Abramson癌症中心（ACC）的共享资源。中国共产党的主要目标是提供熟练的 使用动物疾病模型解释动物病变，以满足ACC成员的需求。之间 提供的服务包括全面的组织学服务，血液和细胞学的临床实验室服务， 免疫组织化学、数字病理学、全面的小鼠表型分型、载玻片评价和 解释、研究设计咨询、尸检培训和显微镜检查。IHC服务 （免疫组织化学）利用自动化平台，多重免疫荧光，原位 CPC具有许多库存优化的抗体，以及处理新的抗体的能力。 研究人员的抗体。CPC在2018年获得了NIH共享仪器补助金（S10），用于 Aperio VERSA扫描仪和eSlide Manager图像分析系统。这个数字扫描仪提供明场 和组织的荧光成像，并与整个载玻片成像软件完全集成， 提供最先进的定量分析Amy达勒姆，MS，VMD，美国大学文凭 兽医病理学家和宾夕法尼亚兽医病理生物学系副教授开发了 领导中国共产党。她是一名委员会认证的解剖兽医病理学家，在以下方面具有丰富的经验： 癌症的动物模型，并参与了许多合作研究项目的成员， ACC. Enrico Radaelli，DVM，PhD，欧洲兽医病理学学院文凭，是CPC 技术总监。他在实验室动物病理学方面有十多年的经验， 临床前/生物医学研究，重点是基因工程小鼠模型的表型分析。 CPC在2018年制定了一项奖学金计划，以扩大服务和使用， 病理学家现在支持CPC的使命。CPC病理学家与多个研究项目合作，包括 肿瘤生物学，癌症治疗学，乳腺癌，免疫生物学，放射生物学和成像。ACC 在最近一次使用共享资源的190名研究者中， 报告所述期间（2018年1月7日至2019年6月30日）。产品总分类有助于为多个高影响力项目提供支持 研究，包括多形性胶质母细胞瘤转化小鼠模型的表征和验证 研究表皮生长因子受体胞外区丙氨酸289位错义突变对 体内（Binder等人，Cancer Cell，2018）。识别和准确解释 动物模型中的组织病理学损伤对于癌症研究的成功至关重要。的利用和 癌症研究中动物模型的复杂性不断增加，CPC也随着这一需求而增长， 扩大其服务的广度和深度，以最好地为行政协调会成员服务。

PROJECT SUMMARY – Comparative Pathology Core The Comparative Pathology Core (CPC) is an established Shared Resource within the Department of Pathobiology at the University of Pennsylvania School of Veterinary Medicine (Penn Vet), and a fully integrated Shared Resource of the Abramson Cancer Center (ACC). The main objective of the CPC is to provide skillful interpretation of lesions in animals to fulfill the needs of ACC members using animal models of disease. Among the services provided are a full-service histology, clinical laboratory services for bloodwork and cytology, immunohistochemistry, digital pathology, comprehensive mouse phenotyping, slide evaluation and interpretation, study design consultation, necropsy training, and photomicroscopy. The IHC service (immunohistochemistry) utilizes an automated platform, multiplex immunofluorescence, and in situ hybridization; the CPC has numerous in-stock optimized antibodies, as well as the ability to work-up novel antibodies for investigators. The CPC was awarded an NIH Shared Instrumentation Grant (S10) in 2018 for the Aperio VERSA scanner and eSlide Manager image analysis system. This digital scanner provides brightfield and fluorescence imaging of tissues and is fully integrated with whole slide imaging software, allowing the CPC to offer state-of-the-art quantitative analysis. Amy Durham, MS, VMD, Diplomate of the American College of Veterinary Pathologists and Associate Professor in the Department of Pathobiology at Penn Vet, developed and directs the CPC. She is a board-certified anatomic veterinary pathologist with considerable experience in animal models of cancer and has been involved in numerous collaborative research projects with members of the ACC. Enrico Radaelli, DVM, PhD, Diplomate of the European College of Veterinary Pathology, is the CPC Technical Director. He has more than a decade of experience in the pathobiology of laboratory animals used in preclinical/biomedical research with an emphasis on the phenotyping of genetically engineered mouse models. The CPC developed a fellowship program in 2018 to expand services and usage, and two additional veterinary pathologists now support the CPC mission. CPC pathologists work with multiple Research Programs, including Tumor Biology, Cancer Therapeutics, Breast Cancer, Immunobiology, and Radiobiology and Imaging. ACC members accounted for 61 of 190 investigators (32%) using the Shared Resource during the most recent reporting period (07/01/18-06/30/19). The CPC is instrumental in providing support for multiple high impact studies, including the characterization and validation of translational mouse models of glioblastoma multiforme to study the impact of epidermal growth factor receptor extracellular domain missense mutations at alanine 289 in vivo (Binder et al., Cancer Cell, 2018). Recognition and accurate interpretation of gross and histopathological lesions in animal models is essential for the success of cancer research. As the use and complexity of animal models in cancer research continues to increase, the CPC has grown with this demand, expanding the breadth and depth of its services to best serve ACC members.