Core B: Biospecimen Core

Biosecimen核心：项目摘要/摘要 我们“3-D衰老和阿尔茨海默病脑计划”的首要目标是定义，使用基于单细胞的 用空间分辨转录组和蛋白质组测量的方法，老化和 阿尔茨海默病(AD)相关脑部三维病理及其相互关系 关于认知功能衰退。实现这一目标的关键是始终如一地获得良好表型、高质量的人类 已同意广泛共享数据的脑组织。Biosecimen(BIO)核心负责 汇编了阿尔茨海默病最大的组织集合之一，这些组织跨越了AD的寿命和已知的病理特征 通过在爱丁堡大学继续收集类似表型的高质量病例， 拉什阿尔茨海默氏症中心和纽约脑库(NYBB)。Bio Core的目标是将 用现代分子技术从脑组织中获取临床病理数据，促进创新 衰老与阿尔茨海默病的研究。生物核心将(1)通过确定以下方式支持四个项目的每一个： 准备并分发每个目标的最佳可用样本，(2)确定每个目标的剖面图 样本，以及(3)从每个样本集合标准化结果测量。Bio Core还将支持 空间多组学(SM)核心的创新方法开发通过收购和 提供高质量的分子级组织。同时，协调的表型数据将流向 集成计算分析(ICA)核心，将使表型与每个项目的数据保持一致。 将使用由行政核心管理的实验室信息管理系统 组织和跟踪所有样本特征，从而能够对样本选择和 准备过程，以发现任何薄弱领域，并确定新的质量措施。总而言之，这本传记 CORE将显著提高本计划产生的数据的质量、注解和可重用性 它的过程和方案将有助于指导和加强更广泛的组织处理方面的改进 神经退行性疾病社区。

BIOSPECIMEN CORE: PROJECT SUMMARY/ABSTRACT The overarching goal of our “3-D Aging & Alzheimer Brain Program” is to define, using single cell-based approaches with spatially-resolved transcriptomic and proteomic measurements, the effect of aging and Alzheimer’s disease (AD)-related pathologies on the brain in three dimensions and the relation of these effects on cognitive decline. Crucial to achieving this goal is consistent access to well-phenotyped, high quality, human brain tissue that has been consented for broad data sharing. The Biospecimen (BIO) Core will be responsible for compiling one of the largest collections of tissue across the lifespan and known pathological hallmarks of AD by continuing prospective collection of comparably phenotyped high quality cases at the University of Edinburgh, the Rush Alzheimer’s Disease Center, and the New York Brain Bank (NYBB). The goal of the BIO Core is to link clinical-pathologic data with contemporary molecular techniques from brain tissue to facilitate innovative research studies on aging and AD. The BIO core will (1) support each of the four Projects by identifying, preparing, and distributing the best available samples for each aim, (2) determine the plane of section for each sample, and (3) assemble standardized outcome measures from each sample. The BIO Core will also support the innovative methodological development of the Spatial Multiomics (SM) Core through the acquisition and provision of high-quality molecular grade tissues. In parallel, the harmonized phenotypic data will flow to the Integrated Computational Analysis (ICA) Core which will align the phenotypes with data for each of the Projects. A Laboratory Information Management System (LIMS), managed by the Administrative Core, will be used to organize and track all sample characteristics, enabling a structured evaluation of the sample selection and preparation process to uncover any areas of weakness and identify new quality measures. In summary, the BIO Core will significantly enhance the quality, annotation, and re-purposability of the data produced by this Program and its processes and protocols will help guide and enhance improvements in tissue processing for the wider neurodegenerative disease community.