STRUCTURE BASED STUDIES OF RNA BINDING PROTEINS FROM HIV

描述：（改编自研究者摘要）研究者 建议使用分子遗传学和核磁共振相结合的方法 光谱探测蛋白质/RNA相互作用的结构基础 并找出新的预防手段。 他提出了一个更 RNA的肽骨架构象的详细分析 HIV达特结合域，用于绘制氨基酰基侧构象 链，并确定其强大的结构基础， 螺旋形成的意想不到的趋势。作者打算 制备和表征由短的生物学上的 活性达特肽与合成的TAR RNA，以评估变化 在RNA和蛋白质构象中， 抑制性药物设计的潜在靶点。 任何人的重要性 观察到的特定化学接触将通过突变 核苷酸或氨基酸参与，然后检查生物和 突变的结构效应。

DESCRIPTION: (Adapted from investigator's abstract) The investigator proposes to use the combined approaches of molecular genetics and NMR spectroscopy to probe the structural basis of protein/RNA interactions and to identify new means of preventing them. He proposes a more detailed analysis of the peptide backbone conformation of the RNA binding domain of HIV Tat to map the conformations of the amino-acyl side chains and to determine the structural basis for its strong and unexpected tendency for helix formation. The author then intends to prepare and characterize the complex formed by a short, biologically active Tat peptide with synthetic TAR RNA in order to assess the changes in RNA and protein conformation that occur upon binding and to identify potential targets for inhibitory drug design. The importance of any specific chemical contacts observed will be tested by mutating the nucleotides or amino acids involved and then examining the biologic and structural effects of the mutations.