OBJECTIVE ANALYSIS OF CHROMOSOME BANDING PATTERNS

该项目旨在开发和商业化显着改善 染色体带型分析和异常检测软件。 与现代计算机化染色体分析一起使用，这将允许 染色体显带的客观表征和方便显示 模式，并将染色体与正常和 不正常的原型和彼此。 这将有助于检测和 量化亚可见带模式改变，特别是高 分辨率条带。 这将大大提高 自动染色体分析仪器，以评估染色体 正常和肿瘤性哺乳动物细胞的改变。 具体来说，我们将使用频繁域特征分析，一个新的图像 贝勒医学院开发的分析技术， 将染色体图像分解为组件，选择那些反映 消除时带型的相关特征 反映预备伪像的成分，染色体间变异， 和其他不相关的差异。 我们将扩展特征分析 软件，以便它可以（1）交互式测试染色体的特定 异常，2）自动筛选染色体为指定的组 （3）通过比较染色体的大小， 每一个与每一个同源物类型的原型，（4）比较同源物与每一个 其他检测微妙的异常和（5）开发原型 显示遗传连锁的人群的染色体图像 并将其与正常人进行比较。 我们将在 PSII的Genetiscan商用自动染色体核型分析工作站。 建议的商业应用：分析开发完成后 并符合常规应用条件，将纳入 PSII的GENETISCAN产品，无论是在新的系统销售，并作为升级 现有的系统。

This project aims to develop and commercialize significantly improved software for chromosome banding pattern analysis and anomaly detection. Used with modern computerized chromosome analysis, this will allow objective characterization and convenient display of chromosome banding patterns, and quantitative comparison of chromosomes to normal and abnormal prototypes and to each other. This will help to detect and quantify sub-visible band pattern alterations, particularly with high resolution banding. This will significantly increase the ability of automated chromosome analysis instruments to evaluate chromosome alterations in normal and neoplastic mammalian cells. Specifically, we will use frequently domain eigenanalysis, a new image analysis technique developed at the Baylor College of Medicine (BCM), to decompose chromosome images into components, selecting those that reflect relevant characteristics of the banding pattern while eliminating components reflecting preparatory artifact, inter- chromosome variation, and other irrelevant differences. We will extend the eigenanalysis software so that it can (1) interactively test chromosomes for specific abnormalities, 2) automatically screen chromosomes for a specified group of abnormalities, (3) automatically classify chromosome by comparing each to the prototype of each homolog type, (4) compare homologs to each other to detect subtle abnormalities and (5) develop prototypical chromosome images for groups of people manifesting genetically linked diseases and compare these with normals. We will implement this on PSII's Genetiscan commercial automatic karyotyping workstation. PROPOSED COMMERCIAL APPLICATION: As soon as the analysis is developed and qualified for routine application, it will be incorporated into PSII's GENETISCAN products, both in new systems sold and as an upgrade to existing systems.