Changeable aspects of radiation-induced chromosome aberrations during early development of the zygote

受精卵早期发育过程中辐射引起的染色体畸变的变化

基本信息

  • 批准号:
    13680615
  • 负责人:
  • 金额:
    $ 2.37万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2004
  • 项目状态:
    已结题

项目摘要

To understand changeable aspects of radiation-induced chromosome aberrations (CAs) during the embryonic development, mouse pie-implantation and post-implantation embryos derived from gamma-irradiated (2,4 Gy) spermatozoa were studied cytogenetically and embryologically.1.Ahighly successful method for chmmosome preparation of mouse 1-cell to 4-cell embryos was established.2.A total number of 1,170 zygotes were karyotyped in irradiated and control groups. The zygotes with structural chromosome aberrations (SCAs) showed a clear dose-dependent increase, reaching 36% in the 4 Gy group. No dose-dependent increase of aneuploidy was found. The most predominant type of CA was chromosome breaks/fragments (60%) which was followed by dicentric chromosomes (25%).3.A total number of 396 2-cell embryos were karyotyped. The incidence of embryos with SCAs was nearly the same as that in 1-cell embryos, but about 90% of them converted into mosaic-type SCA. The incidence of SCA-derived aneuploidy was also significantly high in the irradiated groups.4.A total number of 537 4-cell embryos were karyotyped. A great majority of the embryos with SCA were of mosaic-type. The incidence of aneuploidy became decreased, suggesting that they converted again into mosaic type.5.About 50 to 60% of embryos with SCA reached to blastocyst stage, showing no developmental arrest or delay.6.Chromosome analysis was carried out with 2,240 somatic cells obtained from 85 live embryos (10〜11-day old). None of embryos was found to be chromosomally abnormal. The incidence of post-implantation death was significantly high in the irradiated groups, almost all the dead embryos were being in the state of degenerated conceptus. These suggest that selection of the embryos with CA mainly takes place immediately after implantation.7.No increase of embryonic death or developmental anomalies was observed in 16〜17-day-old fetuses (survey of 52 pregnant females), supporting the above-mentioned suggestion.
为了解辐射诱发的染色体畸变(CA)在胚胎发育过程中的变化情况,本研究从细胞遗传学和胚胎学两个方面研究了γ射线(2,4戈伊)照射后小鼠精子的植入前和植入后胚胎。在照射组和对照组中对170个受精卵进行核型分析。具有结构染色体畸变(SCA)的受精卵显示出明显的剂量依赖性增加,在4戈伊组中达到36%。结果表明,在不同剂量组中,染色体畸变的发生率不同,非整倍体的发生率无剂量依赖性,最常见的畸变类型为染色体断裂/碎片(60%),其次为双着丝粒染色体(25%)。SCA的发生率与1-细胞胚胎的发生率基本相同,但约90%的SCA转化为嵌合型SCA。辐射组中SCA衍生的非整倍体的发生率也显著较高。4.对537个4-细胞胚胎进行了核型分析。绝大多数SCA胚为嵌合型胚。5.SCA胚胎中有50%~ 60%进入囊胚期,未出现发育停滞或延迟。6.对85个10 ~ 11日龄活胚的2,240个体细胞进行染色体分析。未发现胚胎染色体异常。植入后死亡率明显高于对照组,几乎所有死亡胚胎均处于退化的孕体状态。7.16 ~ 17日龄胎儿(52只孕母)的胚胎死亡率和发育异常率均未增加,支持了上述观点。

项目成果

期刊论文数量(23)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Abnormal chromosome migration and chromosome aberrations in mouse oocytes during meiosis II in the presence of topoisomerase II inhibitor ICRF-193
拓扑异构酶 II 抑制剂 ICRF-193 存在下减数分裂 II 期间小鼠卵母细胞的异常染色体迁移和染色体畸变
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Hiroyuki Tateno
  • 通讯作者:
    Hiroyuki Tateno
Avian chromosomes can be examined by injection of erythrocyte nuclei into mouse oocytes
可以通过将红细胞核注射到小鼠卵母细胞中来检查禽类染色体
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Tateno;H. et al.;Hiroyuki Tateno
  • 通讯作者:
    Hiroyuki Tateno
Correlation between the morphology and motility of spermatozoa and the chromosome aberration
精子形态和活力与染色体畸变的相关性
Chromosome analysis of mouse one-cell androgenones derived from a sperm nucleus exposed to topoisomerase II inhibitors at pre-and post-fertilization stages
在受精前和受精后阶段暴露于拓扑异构酶 II 抑制剂的小鼠单细胞雄激素的染色体分析
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Tateno;H. et al.
  • 通讯作者:
    H. et al.
What happens after fertilization of radioactive ray-exposed sperm and oocytes
受放射性射线照射的精子和卵母细胞受精后会发生什么
  • DOI:
  • 发表时间:
    2002
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Kamiguchi;Y.
  • 通讯作者:
    Y.
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KAMIGUCHI Yujiroh其他文献

KAMIGUCHI Yujiroh的其他文献

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{{ truncateString('KAMIGUCHI Yujiroh', 18)}}的其他基金

The correlation between chromosome aberrations and head morphology or motility of human spermatozoa : Analysis by using intracytoplasmic sperm injection into mouse oocytes
染色体畸变与人类精子头部形态或运动力之间的相关性:通过使用胞浆内精子注射到小鼠卵母细胞中进行分析
  • 批准号:
    10470339
  • 财政年份:
    1998
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Effects of neutrons on human sperm chromosomes
中子对人类精子染色体的影响
  • 批准号:
    05454606
  • 财政年份:
    1993
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Effects of radiation and chemicals on human sperm chromosomes
辐射和化学物质对人类精子染色体的影响
  • 批准号:
    02455006
  • 财政年份:
    1990
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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