MECHANISM OF HORMONE ACTION ON CELLS IN CULTURE

乳腺癌是年龄段妇女发病率和死亡率的主要原因 35岁以上关于致癌过程的知识是有限的。 了解雌激素靶细胞增殖的控制是 实施预防、预后、诊断和治疗 旨在降低这些妇女发病率和死亡率的措施。我们 采用了这样一个前提，即扩散是一种构成性的、占主导地位的财产， 后生动物的细胞。因此，我们研究的核心是 乳腺肿瘤增殖特异性抑制剂的鉴定 细胞我们确定人血清白蛋白（HSA）为此类抑制剂。 用重组人血清白蛋白证实其抑制作用是特异性的 关于靶细胞类型;在激素和生长因子中，只有 雌激素克服了这种抑制作用。此外，没有其他蛋白质 产生这种抑制作用。因为人血白蛋白血清 也能够抑制MCF 7细胞的增殖，出现了一个悖论。 我们发现的证据与这一假设相一致， 含有截短的HSA多肽，因此，HSA可以是 被认为是“激素原”。我们将更好地解决这一矛盾， 进一步阐明了HSA和雌激素对 支持期间乳腺肿瘤细胞的增殖。

Breast cancer is a leading cause of morbidity and mortality among women age 35 and older. Knowledge on the carcinogenic process is limited. Understanding the control of the proliferation of estrogen target cells is crucial to implement preventive, prognostic, diagnostic and therapeutic measures aimed at reducing the morbidity and mortality in these women. We adopted the premise that proliferation is a constitutive, dominant property of cells in metazoa. Thus, the centerpiece of our research is the identification of specific inhibitors of the proliferation of breast tumor cells. We identified human serum albumin (HSA) as such type of inhibitor. Using recombinant HSA we verified that the inhibitory effect was specific regarding the target cell type; among hormones and growth factors, only estrogens overcame this inhibitory effect. Moreover, no other protein was found to produce this inhibitory effect. Because human analbuminemic serum is also able to inhibit the proliferation of MCF7 cells, a paradox emerged. We found evidence consistent with the hypothesis that analbuminemic serum contains as truncated HSA polypeptide, and therefore, HSA may may be considered as a "prohormone". We will better to resolve this paradox and shed further light on the mechanism of action of HSA and estrogens on the proliferation of breast tumor cells during the period of support.