QUANTITATION OF ALLELIC IMBALANCE IN SOLID TUMORS

等位基因缺失或“杂合性缺失”（洛），是一种遗传性缺失， 肿瘤细胞中的异常，是肿瘤抑制突变的标志物， 1或基因组不稳定性在人类癌症的发生或发展。 某些遗传区域的等位基因丢失和全基因组指数 等位基因丢失是远处转移的良好预测因子 和生存率，但临床应用受到阻碍， 传统洛丢失方法的几个技术缺陷。整体 本研究的目的是改进测量等位基因丢失的方法 并展示这种测量的实际用途。与使用 基于PCR的微卫星重复多态性和定量磷 影像分析，我们将探讨检测等位基因的可行性， 存档（福尔马林固定，石蜡包埋）临床 前列腺癌标本，以及自动化和规模扩大， 允许快速测量全基因组等位基因丢失的程序。这些 研究可能会导致癌症研究和临床的新应用 药

Allelic loss or "loss of heterozygosity" (LOH), a type of genetic abnormality in tumor cells, is a marker of tumor suppressor mutation and 1 or genomic instability in the genesis or progression of human cancers. Allelic losses of certain genetic regions, and an index of whole-genome allelic loss, have been shown to be good predictors of distant metastasis and survival in colorectal cancer, but clinical application is hindered by several technical drawbacks of traditional LOH methods. The overall objective of this study is to improve methods for measuring allelic loss and to demonstrate practical uses for such measurement. With the use of PcR-based microsatellite repeat polymorphisms and quantitative phosphor imaging analysis, we will investigate the feasibility of detecting allelic imbalances in archival (formalin-fixed, paraffin-embedded) clinical prostate cancer specimens, and of automation and scale-up of such procedures to allow rapid measurement of genome-wide allelic loss. These studies could lead to new applications in cancer research and clinical medicine.