GEL MICRODROP ASSAY FOR SYNCHRONIZED CELL CLONING

这项研究提出了一种新的方法， 高分泌细胞从一个同步的人口使用细胞调节 唾液蛋白（CeReS），一种天然、无毒的细胞表面蛋白， 已经被证明可以可逆地同步细胞， 凝胶微滴（GMD）中的单个同步化细胞。使用模型 系统，我们将证明细胞可以同步，封装， 并测定具有高分泌速率的克隆。GMD流排序将是 用于回收高分泌细胞用于随后的克隆。有 目前没有方法可用于解析个体的分泌速率 在细胞周期的不同点，然后分离， 回收高分泌细胞。 这项研究将为开发细胞同步奠定基础 方法具有广泛的影响，基础研究，以及 生物制药药物发现、开发和制造。期间 在第二阶段，该公司预计将开发一种工艺， 生物制药研究和制造人员可在 许可证或服务费，并组装检测试剂盒 包括微滴制造器、CeReS和试剂， 全世界的研究人员。

The proposed research presents a novel method for recovering individual, high secreting cells from a synchronized population using Cell Regulatory Sialoprotein (CeReS), a natural, non-toxic, cell surface protein which has been shown to reversibly synchronize cells, and then encapsulating individual synchronized cells in gel microdrops (GMDs). Using a model system, we will demonstrate that cells may be synchronized, encapsulated and assayed for clones with high secretion rates. GMD flow sorting will be used to recover the high secreting cells for subsequent cloning. There is no method currently available for resolving secretion rates of individual cells at various points in the cell cycle, and then isolating and recovering high secreting cells. This research will form the basis for developing a cell synchronization method with broad implications for basic research, as well as for biopharmaceutical drug discovery, development, and manufacturing. During Phase Il, the company expects to develop a process which will be made available to biopharmaceutical research and manufacturing personnel on either a license or fee for service basis and to assemble test kits comprised of a microdrop maker, CeReS and reagents which can be sold to researchers worldwide.