MEASUREMENT OF DNA ADDUCTS IN HUMAN SAMPLES

描述（改编自申请人摘要）：研究者 开发了一种测量N7-鸟嘌呤（N7-G）DNA加合物的方法 环氧乙烷和苯乙烯。 由于环氧乙烷和苯乙烯是 在工业中广泛使用并且在动物中是致瘤的， 研究人员建议将该方法应用于工人的血液样本， 职业性接触这些物质 如果这样的DNA加合物可以 那么该项目可能会导致更好地了解在哪里设置 职业接触这些化学品的限制。 在他们声称既方便又强大的方法中， N7-G加合物通过简单地将DNA加热到室温而容易地从DNA中收获。 水 然后使用简单的试剂和步骤进行衍生和纯化 加合物，然后通过质谱法检测。 相同的方法 在将来可以用于检测其它简单的烷基， 由工业或环境化学品形成的羟烷基N7-G加合物 而不是环氧乙烷或苯乙烯。

DESCRIPTION (Adapted from the Applicant's Abstract): The investigators have developed a method for measuring the N7-guanine (N7-G) DNA adducts of ethylene oxide and styrene. Since ethylene oxide and styrene are widely used in industry and are tumorigenic in animals, the investigators propose to apply the method to blood samples of workers exposed to these agents occupationally. If such DNA adducts can be then the project may lead to a better understanding of where to set occupational exposure limits for those working with these chemicals. In their method, which they claim is both convenient and powerful, the N7-G adducts are easily harvested from DNA by simply heating the DNA in water. Simple reagents and steps are then used to derivatize and purify the adducts prior to detection by mass spectrometry. The same method can in the future find use in the detection of other simple alkyl and hydroxalkyl N7-G adducts formed by industrial or environmental chemicals other than ethylene oxide or styrene.