ADHERENCE OF PERIODONTAL DISEASE-ASSOCIATED BACTERIA

最近的临床研究强烈表明， 中间普雷沃氏菌，一种革兰氏阴性黑色素 专性厌氧杆，随着牙周病的发展。这 微生物经常从牙周病损中分离出来， 各种形式的牙周炎，并且通常被认为是 牙周病微生物人们认识到，对于这种微生物， 在宿主体内存活并成为潜在的病原体， 先决条件是它能够粘附到宿主组织或其他组织上， 细菌牙周病损中经常分离到中间型巴斯德菌 这表明，这种生物可能具有粘附和 定殖于宿主组织。然而，关于信徒的情况知之甚少。 可能与这种龈下细菌有关的特性。 在我们早期对P. intermedia口服菌株的观察中， 在这些生物体上观察到表面附属物（菌毛）。分离 来自这些细菌的菌毛能够结合各种哺乳动物 红细胞，粘附活性的表达。工作假设 因此，这项拟议研究的目的是确定 中间疫霉菌毛可能与中间疫霉的致病性有关 通过介导该生物体对宿主细胞的粘附来调节该生物体。很长的- 本研究的主要目的是：（1）明确P. （2）建立中间宿主细胞的功能; 通过生成介导生物体粘附的组分 缺乏粘附组分的同基因突变体;（3）测试所述突变体中的粘附组分， 通过中间青霉野生型细胞的体内龈下定植与 突变体;和（4）测试天然或重组粘附素作为疫苗， 预防、消除或减少龈下裂隙的感染 松鼠猴拟议工作的具体目标是：（1） 纯化和表征介导该反应的粘合剂组分， 中间青霉细胞与宿主细胞的结合;（2）鉴定中间青霉细胞中的 粘合剂组分的功能域，并表征粘合剂组分的功能域。 使用针对粘附素的单克隆抗体的分子;（3） 以表征导致 粘附分子与宿主细胞的结合;和（4）克隆 编码所述粘附分子的基因并表征所述基因，以及 产品（S）。这些研究的结果应该提供信息， 关于促进龈下炎症的分子机制， P. intermedia的殖民地。

Recent clinical studies have strongly suggested that there is a correlation of Prevotella intermedia, a gram-negative black-pigmented obligate anaerobic rod, with the development of periodontal disease. This organism is frequently isolated from periodontal lesions associated with various forms of periodontitis and is generally accepted as a putative periodontopathic organism. It is recognized that for this microorganism to survive and emerge as a potential pathogen in a host, one of the prerequisites is its ability to adhere to host tissues or to other bacteria. The frequent isolation of P. intermedia from periodontal lesions suggested that this organism may possess the ability to adhere and to colonize host tissues. However, little is known regarding the adherent properties that may be associated with this subgingival bacterium. In our earlier observations of oral strains of P. intermedia, unique surface appendages (fimbriae) were observed on these organisms. Isolated fimbriae from these bacteria were capable of binding to various mammalian erythrocytes, an expression of adherence activity. The working hypothesis of this proposed study therefore is to determine whether or not the fimbriae of P. intermedia may contribute to the pathogenicity of this organism by mediating adherence of this organism to host cells. The long- term objectives are: (1) to determine the adherence mechanisms of P. intermedia to host cells; (2) to establish the function of the component(s) which mediate adherence of the organism by generating isogenic mutants which lack the adhesive component(s); (3) to test the in vivo subgingival colonization by P. intermedia wild type cells versus the mutants; and (4) to test the native or recombinant adhesin as a vaccine to prevent, eliminate, or reduce infection in the subgingival crevice of squirrel monkeys. The specific aims of the proposed work will be: (1) to purify and characterize the adhesive component(s) which mediate the binding of P. intermedia cells to host cells; (2) to identify the functional domain(s) of the adhesive component(s) and to characterize the molecule(s) using monoclonal antibodies raised against the adhesin(s); (3) to characterize the morphological and biochemical events leading to the binding of the adhesive molecule(s) to host cells; and (4) to clone gene(s) encoding the adhesive molecule(s) and characterize the gene(s) and their product(s). The results of these studies should provide information regarding the molecular mechanisms which would promote subgingival colonization by P. intermedia.