ADHERENCE OF PERIODONTAL DISEASE-ASSOCIATED BACTERIA

最近的临床研究强烈表明，有一种 革兰氏阴性黑色素中间普氏杆菌的相关性研究 专性厌氧棒，随着牙周病的发展。这 经常从牙周病变中分离出与以下相关的微生物 各种形式的牙周炎，通常被认为是 牙周病有机体。人们认识到，对于这种微生物来说， 在宿主体内存活并作为潜在病原体出现，其中一种 先决条件是它能够附着在宿主组织或其他组织上 细菌。牙周病变中常见的中间肺炎杆菌的分离 提示这种有机体可能具有黏附和 定植宿主组织。然而，关于追随者的情况却知之甚少。 可能与这种龈下细菌有关的特性。 在我们早先对中间肺炎杆菌口腔菌株的观察中， 在这些生物体上观察到表面附属物(菌毛)。孤立 这些细菌的菌毛能够与各种哺乳动物结合 红细胞，一种黏附活性的表达。工作假说 因此，这项拟议研究的目的是确定 中间拟青霉菌毛可能与其致病性有关。 通过调节这种有机体与宿主细胞的黏附来实现的。长的- 任期目标是：(1)确定P。 中间体到宿主细胞；(2)建立 成分(S)，通过生成物质来调节有机体的黏附 缺乏粘连成分的同基因突变体(S)；(3)检测 中间假单胞菌野生型细胞在牙周组织中的定植 (4)将天然或重组粘附素作为疫苗进行试验 预防、消除或减少牙龈下缝隙中的感染 松鼠猴子。拟议工作的具体目标是：(1) 细胞黏附性成分(S)的纯化与鉴定 中间假单胞菌与宿主细胞的结合；(2)鉴定 粘合剂成分(S)的功能域(S)，并表征 使用针对粘附素的单克隆抗体制备的分子(S)；(3) 来描述导致这些疾病的形态和生化事件 黏附分子(S)与宿主细胞的结合；(4)克隆 编码黏附分子的基因(S)和特征基因(S)和 他们的产品(S)。这些研究的结果应该提供信息 关于促进龈下生长的分子机制 中间假单胞菌的定殖。

Recent clinical studies have strongly suggested that there is a correlation of Prevotella intermedia, a gram-negative black-pigmented obligate anaerobic rod, with the development of periodontal disease. This organism is frequently isolated from periodontal lesions associated with various forms of periodontitis and is generally accepted as a putative periodontopathic organism. It is recognized that for this microorganism to survive and emerge as a potential pathogen in a host, one of the prerequisites is its ability to adhere to host tissues or to other bacteria. The frequent isolation of P. intermedia from periodontal lesions suggested that this organism may possess the ability to adhere and to colonize host tissues. However, little is known regarding the adherent properties that may be associated with this subgingival bacterium. In our earlier observations of oral strains of P. intermedia, unique surface appendages (fimbriae) were observed on these organisms. Isolated fimbriae from these bacteria were capable of binding to various mammalian erythrocytes, an expression of adherence activity. The working hypothesis of this proposed study therefore is to determine whether or not the fimbriae of P. intermedia may contribute to the pathogenicity of this organism by mediating adherence of this organism to host cells. The long- term objectives are: (1) to determine the adherence mechanisms of P. intermedia to host cells; (2) to establish the function of the component(s) which mediate adherence of the organism by generating isogenic mutants which lack the adhesive component(s); (3) to test the in vivo subgingival colonization by P. intermedia wild type cells versus the mutants; and (4) to test the native or recombinant adhesin as a vaccine to prevent, eliminate, or reduce infection in the subgingival crevice of squirrel monkeys. The specific aims of the proposed work will be: (1) to purify and characterize the adhesive component(s) which mediate the binding of P. intermedia cells to host cells; (2) to identify the functional domain(s) of the adhesive component(s) and to characterize the molecule(s) using monoclonal antibodies raised against the adhesin(s); (3) to characterize the morphological and biochemical events leading to the binding of the adhesive molecule(s) to host cells; and (4) to clone gene(s) encoding the adhesive molecule(s) and characterize the gene(s) and their product(s). The results of these studies should provide information regarding the molecular mechanisms which would promote subgingival colonization by P. intermedia.