BLADDER SMOOTH MUSCLE CELL RESPONSE TO MECHANICAL FORCES

描述：(改编自申请人的摘要)长期 这项应用的目标是了解膀胱的机制 平滑肌细胞对机械信号做出反应，这是机械信号的一部分 它们在体内的直接环境。一台仪器已经被 开发了允许应用精确水平的Biaxal 在培养过程中对细胞的变形。这种机械装置的影响 菌株可以从形态、生化和 生理上的。据推测，拉伸或拉伸引起的压力 静水压力改变膀胱平滑肌细胞 这种变形的结果是结缔组织合成的改变。 这会导致膀胱顺应性变差。的具体目标 本研究的建议是：(1)利用膀胱建立体外模型 并使用拉伸使这些细胞机械变形 和或静水压力；以及(2)确定基质合成是否通过 张力与非张力作用下膀胱平滑肌细胞的变化 条件。

DESCRIPTION: (Adapted from the applicant's abstract) The long term goal of this application is to understand the mechanism by which bladder smooth muscle cells respond to mechanical signals which are a part of their immediate in vivo environment. An apparatus has been developed which permits the application of precise levels of biaxal deformation to cells in culture. The effects of this mechanical strain can be assessed morphologically, biochemically, and physiologically. It is hypothesized that stress induced by stretch or hydrostatic pressure alters bladder smooth muscle cells and that one result of this deformation is an alteration in connective tissue synthesis which causes the bladder to become less compliant. The specific aims of this proposal are: (1) to set up an in vitro model using bladder smooth muscle cells and to mechanically deform these cells using stretch and or hydrostatic pressure; and (2) to determine if matrix synthesis by bladder smooth muscle cells is altered under strained and unstrained conditions.