MODEL POLYMERS FOR TISSUE ENGINEERING

细胞移植疗法，将分离的细胞附着在 植入宿主体内的支架，对器官有吸引力 置换和组织修复治疗多种医疗疾病，包括 糖尿病、肝功能衰竭和皮肤损伤。的最新发展 聚(乳酸-共赖氨酸)(PLAL)，Barrera等人。(1993)代表 朝着获得细胞的理想矩阵迈出的潜在巨大一步 移植。PLAL是一种含有赖氨酸的可生物降解的共聚物 可以用所需的配体修饰的残基。因此，共轭 PLAL对各种生物因素的研究为精确度打开了大门 对材料进行工程设计以引起特定的医学反应。在 目前的建议，研究针对的是表征细胞 与多肽偶联PLAL的相互作用。与以下各项对应的多肽 在纤维连接蛋白和层粘连蛋白中发现的附着因子将被检测 它们能够促进细胞的黏附、迁移、分化和/或 几种不同类型的细胞的增殖，包括成纤维细胞和 肝细胞。将使用图像分析方法来监控迁移 以及细胞生物学和生化技术将被调用 检测细胞增殖、黏附和分化情况。此外, 将详细探索多肽与PLAL的偶联以确定理想的 多肽浓度、交联剂化学和最佳长度 在多肽和聚合物之间。这项工作应该有助于我们深入了解 通过它可以重现天然生物基质蛋白的作用。 从这项工作中，支架可以设计成临时的 细胞外基质引导细胞合成自身基质 以及重组所需的组织。

Cell transplantation therapy, in which isolated cells are attached to a scaffold for implantation into a host, is attractive for organ replacement and tissue repair for numerous medical disorders including diabetes, liver failure and skin damage. The recent development of poly(lactic acid-co-lysine) (PLAL) by Barrera et al. (1993) represents a potentially enormous step toward attaining the ideal matrix for cell transplantation. PLAL is a biodegradable copolymer containing lysine residues that can be modified with desired ligands. Thus, the conjugation of PLAL to various biological factors opens the door for precision engineering of materials to elicit specific medical responses. In the current proposal, research is directed toward characterizing cell interactions with peptide-coupled PLAL. Peptides corresponding to attachment factors found in fibronectin and laminin will be assayed for their ability to promote cell adhesion, migration, differentiation and/or proliferation of several diverse cell types, including fibroblasts and hepatocytes. Image analysis approaches will be used to monitor migration of cells, and cell biological and biochemical techniques will be invoked to measure proliferation, adhesion and differentiation. In addition, peptide coupling to PLAL will be explored in detail to determine ideal peptide concentrations, cross-linking chemistry, and optimal length between the peptide and polymer. This work should shed insights into ways by which effects of natural biological matrix proteins can be reproduced. From this work, supports can be designed to serve as a temporary extracellular matrix to guide cells toward synthesizing their own matrix and restructuring a desired tissue.