FETAL GASTRIC EPITHELIAL CELLULAR DEVELOPMENT

这一提议检验了胎儿胃上皮细胞 生长发育在很大程度上依赖于营养的管腔作用。 羊水中存在的物质。初步研究表明，当 在妊娠晚期防止羊水吞咽。 在体胎兔经食道结扎后，明显抑制 胃的形态和功能发生发育。体外羊水 液体对培养的胎儿胃上皮细胞有营养作用。这个 该项目的具体目标是：1)检查已知的 羊水中发现的营养物质对胎儿胃发育的影响 活体上皮；2)检查已知营养剂在体内的影响 羊水对体外培养胎儿胃上皮细胞生长的影响； 3)初步分离羊水中的营养因子 体外模型和体内模型作为生物检测方法。胃的发育 将通过光和电子对上皮细胞进行形态计量评估 显微镜下观察，G细胞免疫反应染色。功能性 在体内的发育将通过测量胃酸和 胃蛋白酶分泌和血清胃泌素水平。潜在的重要营养 有待研究的因素包括那些已知会影响新生儿和成人的因素。 胃肠生长，包括：腔内营养素、胃泌素、表皮 生长因子、肠高血糖素和皮质醇。亲和层析将 用于按顺序选择性去除羊水中的营养物质 以评估它们的相对重要性。另外，羊水会被 高效液相色谱分离和营养成分 每一组分的活性将在体外和体内进行测试。 生物检测。具有高活性的馏分将被表征为 初步的方法。这项工作在健康方面具有直接的意义 儿童发育异常的认识、预防和治疗 胃肠道。

This proposal tests the hypothesis that fetal gastric epithelial development is critically dependent on the luminal action of trophic substances present in amniotic fluid. Preliminary studies show that when swallowing of amniotic fluid during the third trimester is prevented in vivo by esophageal ligation in the fetal rabbit, marked inhibition of morphologic and functional gastric development occurs. In vitro amniotic fluid has a trophic effect on cultured fetal gastric epithelial cells. The specific aims of this project are to: 1) examine the effects of known trophic agents found in amniotic fluid on the development of fetal gastric epithelium in vivo; 2) examine the effect of known trophic agents found in amniotic fluid on the growth of fetal gastric epithelial cells in vitro; and 3) isolate in a preliminary way trophic factors in amniotic fluid using the in vitro and in vivo models as bioassays. Development of gastric epithelial cells will be assessed morphometrically by light and electron microscopy, and immunoreactive staining for G-cells. Functional development will be assessed in vivo by measurements of gastric acid and pepsin secretion, and serum gastrin levels. Potentially important trophic factors to be studied include those known to affect neonatal and adult gastrointestinal growth, including: luminal nutrients, gastrin, epidermal growth factor, enteroglucagon, and cortisol. Affinity chromatography will be used to selectively remove trophic agents from amniotic fluid in order to assess their relative importance. In addition, amniotic fluid will be fractionated by high performance liquid chromatography and the trophic activity of each fraction will be tested in the in vitro and in vivo bioassays. Fractions with high activity will be characterized in a preliminary way. This work has direct health-related significance in the understanding, prevention, and treatment of developmental anomalies of the gastrointestinal tract.