Maximising synthetic peptide and protein manufacture by in vivo DNA assembly in bacteria using high throughput robotics

许多不同的因素会影响一段DNA是否能在生物环境中工作，以表达它编码的蛋白质。蛋白质代表了一大类令人兴奋但昂贵的新药。当蛋白质制造商需要通过工业生产大量生产时，DNA活性低下尤其成问题。在牛津遗传学公司，我们开发了广泛的工具和专业知识，使我们能够设计和构建产生大量蛋白质的DNA序列。在这个项目中，我们的目标是将现有工作流程的很大一部分工业化，并使复杂DNA的组装成为自动化的高通量过程。这将使快速和有效地开发出最适合在任何系统中生产蛋白质的DNA序列，并应导致蛋白质制造的重大改进。这将使英国商业和医药的许多方面受益。

Many different factors influence whether a piece of DNA will work in a biological setting, to express the protein it encodes. Proteins represent a broad new class of exciting but expensive new medicines. Poor DNA activity is particularly problematic when manufacturers of proteins need to produce large quantities by industrial manufacture. At Oxford Genetics we have developed a wide range of tools and expertise to allow us to design and build DNA sequences that produce high amounts of proteins. In this project we aim to industrialise a large proportion of our existing work flow and make the assembly of complex DNA an automated high-throughput process. This will enable the rapid and efficient development of DNA sequences that are optimal for producing proteins in any system, and should lead to major improvements in protein manufacture. This will benefit many aspects of commerce and medicine in the UK.