EARLY CELLULAR AND MOLECULAR EVENTS IN UVEITIS

描述（改编自申请人的摘要）：葡萄膜炎是主要的 这是美国法律的盲目性的原因。 初始解释 葡萄膜炎发展中的事件可以提供新的治疗方法， 这种致盲性疾病的管理方法。 这些研究 是基于骨髓来源的细胞启动 葡萄膜炎通过将视网膜抗原呈递给CD 4 + T细胞， 骨髓来源的APC可以存在于视网膜和/或脉络膜中。 这 假设将使用骨髓嵌合体进行测试， S抗原特异性T细胞系的转移。 具体而言是 应用将：（1）确定骨髓源性细胞的作用 在实验性自身免疫性葡萄膜视网膜炎（EAU）中， （2）识别和定位骨髓源性T细胞， 视网膜和脉络膜中的细胞，以及（3）确定 这些细胞在葡萄膜炎的发展过程中。 这将是 由一组具有以下专长的调查人员完成： 免疫病理学、免疫组织化学、原位分子技术和 分子生物学

DESCRIPTION (Adapted from applicant's abstract): Uveitis is the leading cause of legal blindness in the United States. Elucidation of initial events in the development of uveitis could provide new therapeutic approaches to the management of this blinding disease. These studies are based on the hypothesis that bone marrow-derived cells initiate uveitis by presenting retinal antigens to CD4+T-cells and such bone marrow-derived APCs may be present in the retina and/or choroid. This hypothesis will be tested using bone marrow chimeras with adoptive transfer of S-antigen specific T-cell lines. Specifically, the application will: (1) determine the role of bone marrow-derived cells in experimental autoimmune uveoretinitis (EAU) by transfer of specific T-cells to chimeras, (2) identify and localize the bone marrow-derived cells in retina and choroid and (3) determine the cytokine profile of these cells during the development of uveitis. This will be accomplished by a team of investigators with expertise in immunopathology, immunohistochemistry, in situ molecular techniques and molecular biology.