Cell-Sort MultiTool: a Novel Platform for Bacterial Single-Cell Analysis

该项目旨在利用整合微流体、显微镜和基因组学的创新方法，在单细胞水平上推进细菌种群抗生素耐药性的分子理解。对单个细菌细胞遗传学的研究对于提供异质性耐药的基本见解至关重要，异质性耐药是耐药发展的重要组成部分。预期结果包括高通量多路筛选的新平台技术和改进的细菌异质性知识，为抗生素的使用提供信息。这一跨学科项目将通过降低医疗保健费用、促进澳大利亚人的健康和将先进技术商业化，在社会和经济方面产生重大效益。

This project aims to advance molecular understanding of antibiotic resistance in bacterial populations at the single-cell level, using an innovative approach integrating microfluidics, microscopy and genomics. The study of individual bacterial cell genetics is essential to provide fundamental insights into heterogeneous resistance, an important component of resistance development. Expected outcomes include a new platform technology for high-throughput multiplexed screening and improved knowledge of bacterial heterogeneity, informing antibiotic usage. This interdisciplinary project should yield significant benefits in society and economy by reducing healthcare costs, boosting health for Australians and commercialising advanced technologies.