CONTROL OF SIGMA BETA ACTIVITY IN B SUBTILIS

αβ是枯草芽孢杆菌的次要因子。阿尔法·贝塔- 含有RNA聚合酶识别几个基因的启动子，这些基因 在指数生长期末期和“热休克”期间表达。 αb的活性受到一系列不同寻常的负离子的调节。 一种调节剂，包括一种结合到 Alphab并阻断其与RNA聚合酶结合的能力。这个 调节子由三个基因(rsbV、rsbW和rsbX)编码，它们是 与Alphab结构基因共转录。我们建议确定 这些监管机构与其 目标(S)，影响他们控制的机制和 它们会对信号做出反应。Alphab及其受体的免疫学探针 已经开发了调节器，并将用于监控其 丰富性、潜在的状态变化和联想。受监管 依赖于字母B的转录将在体外重组并用于 直接测试监管者和他们的目标之间的互动。 RsbV、W、X系统将以各种组合和 在体内通过放置一个或多个依赖转录和 其他监管机构的状况。使用不适当的激活 以α依赖报告基因为筛选标准，基因突变 谁的产品会影响rsbV，W，X体系的活性 隔离和分析以确定信号的性质 RsbV、W和X做出响应。最后，转座子生成的 报告基因融合将被用于鉴定和表征 由字母系统控制的附加操纵子。控制 辅助α因子的活性对正常细胞至关重要 成长。字母b的不适当激活会导致立即和 其调节子的致死性诱导。一个特别耐人寻味的方面 α控制是由反西格玛因子调节的。反阿尔法 因子代表了原核生物中一类“新的”调节分子。 他们的行动模式、监管和他们回应的信号 都是未知的。拆解他们的财产不仅会提供新的 对全球基因控制的见解，但也可能定义不可预见的目标 用于抗菌剂。

AlphaBeta is a secondary alpha factor of Bacillus subtilis. Alpha Beta- containing RNA polymerase recognizes the promoters of several genes that are expressed at the end of exponential growth and during "Heat Shock". The activity of alphaB is modulated by an unusual cascade of negative regulators which includes an anti-sigma protein (RsbW) that binds to alphaB and blocks its ability to associate with RNA polymerase. The regulators are encoded by three genes (rsbV, rsbW and rsbX) that are cotranscribed with the alphaB structural gene. We propose to determine the functional relationships between these regulators and their target(s), the mechanism by which the effect their control and the signals to which they respond. Immunological probes for alphaB and its regulators have been developed and will be used to monitor their abundance, potential change of state, and associations. Regulated alphaB-dependent transcription will be reconstituted in vitro and used to directly test the interactions among the regulators and their targets. The rsbV, W, X system will be reconstituted in various combinations and ratios in vivo by placing one or more of the dependent transcription and the state of the other regulators. Using inappropriate activation of a alpha-dependent reporter gene as a screening criteria, mutations in genes whose products influence the activity of the rsbV, W, X system will be isolated and analyzed to determined the nature of the signals to which RsbV, W, and X respond. Finally a transposon-generated collection of reporter gene fusions will be used to identify and characterize additional operons that are controlled by the alphaB system. Control of the activity of ancillary alpha factors is critical to normal cell growth. Inappropriate activation of alphaB leads to an immediate and lethal induction of its regulon. A particularly intriguing aspects of alphaB control is its regulation by anti sigma factor. Anti-alpha factors represent a "new class" of regulatory molecules in prokaryotes. Their mode of action, regulation and the signals to which they respond are unknown. Unraveling their properties will not only provide new insights for global gene control but may also define unforeseen targets for antimicrobial agents.