ESCHERICHIA COLI 0157--H7 ADHERENCE AND COLONIZATION

性状（改编自申请人摘要）：大肠埃希菌 0157：H7正在成为一种重要的人类胃肠道病原体。 这种微生物会导致腹泻、出血性结肠炎， 尿毒症综合征（HUS）。 HUS是急性肾功能衰竭最常见的原因。 在北美，儿童期失败的发生率正在增加， 似乎是慢性肾损伤的重要原因。 机制 其中E.大肠杆菌0157：H7在人体胃肠道定植 而牛，它的蓄水池，是未知的。 该项目致力于通过以下方式识别和表征粘附素： 其中E.大肠杆菌0157：H7粘附于上皮细胞，这将导致 更好地了解胃肠道定植的生物学 被这种病原体感染 该项目的长期目标是 预防或改善E.大肠杆菌0157：H7感染。 的PI 将试图通过防止殖民化来实现这一目标， 促进E. 大肠杆菌0157：H7 还有人类 为了实现这些目标，他们建议：（1）确认 他们克隆和表达的分子是E. coli O157：H7中表达，（2）研究该粘附素在诱导大肠杆菌O157：H7 在兔结肠炎，并在胃肠道定植 （3）确定该粘附素是否介导E.杆菌 0157：H7感染人体胃肠道组织，并确定是否感染 人类产生这种分子的抗体，（4）研究这种分子 该粘附素在E. coli0157：H7。

DESCRIPTION (Adapted from the applicant's abstract): Escherichia coli 0157:H7 is emerging as an important human gastrointestinal pathogen. This organism causes diarrhea, hemorrhagic colitis, and the hemolytic uremic syndrome (HUS). HUS is the most common cause of acute renal failure in childhood in North America, is increasing in incidence, and appears to be an important cause of chronic renal damage. The mechanism whereby E. coli 0157:H7 colonizes the gastrointestinal tracts of humans and cattle, its reservoir, is unknown. This project endeavors to identify and characterize the adhesin through which E. coli 0157:H7 adheres to epithelial cells, which will lead to a better understanding of the biology of gastrointestinal colonization by this pathogen. The long term objective of this project is the prevention or improved treatment of E. coli 0157:H7 infections. The PI will attempt to achieve this objective by preventing colonization or promoting clearance of E. coli 0157:H7 from mucosal surfaces of cattle and humans. To accomplish these objectives, they propose to (1) confirm that the molecule they have cloned and expressed is the adhesin of E. coli O157:H7, (2) study the role of this adhesin in the induction of colitis in rabbits, and in colonization of the gastrointestinal tract of cattle, (3) determine if this adhesin mediates attachment of E. coli 0157:H7 to human gastrointestinal tissue, and determine if infected humans develop antibodies to this molecule, and (4) study the molecular mechanisms underlying the expression of this adhesin in E. coli 0157:H7.